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Order Code BNP B-Type Natriuretic Peptide (NT-proBNP),  Plasma or Serum

Specimen Requirements

Collection Container/Tube:

Preferred: Green-top (lithium heparin) gel tube.

Acceptable: Plain, red-top tube or a serum gel tube

Specimen Volume: 2 mL

Pediatric (minimum) volume: 0.5 mL in a green top (lithium heparin) MICROTAINER gel tube

Specimen Minimum Volume

0.5 mL

Day(s) Test Set Up

Monday through Sunday

Reference Values

Decision threshold

<75 years: 125 pg/mL

>75 years: 450 pg/mL

Clinical correlations for the New York Heart Association (NYHA) functional classes at the 95th percentile:

NYHA I: 3,410 pg/mL

NYHA II: 6,567 pg/mL

NYHA III: 10,449 pg/mL

NYHA IV: 12,188 pg/mL

Performing Laboratory

Ridgeview Medical Center Laboratory

Two Twelve Medical Center Laboratory

Sibley Medical Center



Specimen Transport Temperature


Plasma/Serum Ambient 3 days
  Refrigerated (preferred) 5 days
  Frozen 12 months


Test Classification and CPT Coding


Useful For

Diagnosis and assessment of individuals suspected of having congestive heart failure or acute coronary syndrome