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Order Code BNP B-Type Natriuretic Peptide (NT-proBNP),  Plasma or Serum

Specimen Requirements

Collection Container/Tube:

Preferred: Green-top (lithium heparin) gel tube.

Acceptable: Plain, red-top tube or a serum gel tube

Specimen Volume: 2 mL

Pediatric (minimum) volume: 0.5 mL in a green top (lithium heparin) MICROTAINER gel tube

Specimen Minimum Volume

0.5 mL

Performing Laboratory

Ridgeview Medical Center Laboratory

Two Twelve Medical Center Laboratory

Sibley Medical Center

Day(s) Test Set Up

Monday through Sunday



Test Classification and CPT Coding


Specimen Transport Temperature


Plasma/Serum Ambient 3 days
  Refrigerated (preferred) 5 days
  Frozen 12 months


Useful For

Diagnosis and assessment of individuals suspected of having congestive heart failure or acute coronary syndrome

Reference Values

Decision threshold

<75 years: 125 pg/mL

>75 years: 450 pg/mL

Clinical correlations for the New York Heart Association (NYHA) functional classes at the 95th percentile:

NYHA I: 3,410 pg/mL

NYHA II: 6,567 pg/mL

NYHA III: 10,449 pg/mL

NYHA IV: 12,188 pg/mL