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Order Code EGFRR Lung Cancer, EGFR with ALK Reflex, Tumor


Specimen Required


Pathology report must accompany specimen in order for testing to be performed.

 

Preferred:

Specimen Type: Tissue

Container/Tube: Tissue block

Collection Instructions: Submit a formalin-fixed, paraffin-embedded tissue block.

 

Acceptable:

Specimen Type: Tissue

Container/Tube: Slides

Specimen Volume: 12 unstained, positively charged, unbaked slides or 2 hematoxylin and eosin-stained slides (will not be returned) and 10 unstained, positively charged, unbaked slides

Collection Instructions: Submit 12 unstained, positively charged, unbaked slides cut at 5-microns or 2 hematoxylin and eosin-stained slides and 10 unstained, positively charged, unbaked slides with 5-micron thick sections of the tumor tissue.


Useful For

Identifying non-small cell lung cancers that may benefit from treatment with epidermal growth factor receptor-tyrosine kinase or anaplastic lymphoma kinase inhibitors

Additional Tests

Test ID Reporting Name Available Separately Always Performed
SLIRV Slide Review in MG No, (Bill Only) Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
LCAF ALK (2p23), Lung Cancer, FISH, Ts Yes No

Testing Algorithm

When this test is ordered, EGFR and LCAF testing will always be ordered. The EGFR Gene, Mutation Analysis, 29 Mutation Panel, Tumor, will always be performed. All specimens without an EGFR mutation will be automatically reflexed to LCAF / ALK (2p23), Lung Cancer, FISH Tissue. Specimens with an identified EGFR mutation will result in cancellation of the LCAF test.

 

When this test is ordered, slide review will always be performed at an additional charge.

Method Name

Polymerase Chain Reaction (PCR) and Fluorescence In Situ Hybridization (FISH)

Reporting Name

Lung Cancer, EGFR with ALK Reflex

Specimen Type

Varies

Specimen Minimum Volume

Formalin-fixed, paraffin-embedded tissue block (preferred) or 2 slides stained with hematoxylin-and-eosin and 10 unstained, positively charged, unbaked slides with 5-micron thick sections of the tumor tissue.

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Frozen 
  Refrigerated 

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Specimens that have been decalcified (all methods); specimens that have not been formalin-fixed, paraffin-embedded; bone marrow in EDTA

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Monday through Friday; 10 a.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration

CPT Code Information

EGFR Gene, Mutation Analysis, Tumor

81235-EGFR (epidermal growth factor receptor) (eg, non-small cell lung cancer) gene analysis, common variants (eg, exon 19 deletions, L858R, T790M, G719S, L861Q)

 

Additional Tests:

Slide Review

88381-Microdissection, manual

 

Reflexed Tests (if appropriate):

Lung Cancer, ALK (2p23), FISH, Ts

88271 x 2-DNA Probe (if appropriate)

88274-Interphase in situ hybridization (if appropriate)

88291-Interpretation and report (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
EGFRR Lung Cancer, EGFR with ALK Reflex 21666-5

 

Result ID Test Result Name Result LOINC Value
53240 Result Summary 50397-9
53241 Result 21666-3
53242 Interpretation 69047-9
53243 Specimen 31208-2
53244 Source 31208-2
54441 Tissue ID 80398-1
53245 Released By 18771-6