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Order Code LEIDE Factor V Leiden (R506Q) Mutation, Blood

Additional Codes

Mayo Test ID
F5DNA

Reporting Name

Factor V Leiden (R506Q) Mutation, B

Useful For

Patients with clinically suspected thrombophilia and: 1) activated protein C (APC)-resistance either proven or suspected by a low or borderline APC-resistance ratio, or 2) a family history of factor V Leiden

Specimen Type

Whole blood


Advisory Information


Coagulation-based activated protein C (APC)-resistance ratio (mixing with factor V-deficient plasma) is recommended as the initial screening assay for APC-resistance. Depending on the assay system, the APC-resistance ratio may be indeterminate for patients with a lupus anticoagulant or extremely high heparin levels. For more information, see APCRV / Activated Protein C Resistance V (APCRV), Plasma or APCRR / Activated Protein C Resistance V (APCRV), with Reflex to Factor V Leiden, Plasma.



Specimen Required


Container/Tube: 

Preferred: Yellow top (ACD)

Acceptable: EDTA or sodium citrate

Specimen Volume: Full tube

Collection Instructions: 

1. Invert several times to mix blood.

2. Send specimen in original tube.

Additional Information: Can be combined with other molecular coagulation tests;

-MTHAC / 5,10-Methylenetetrahydrofolate Reductase A1298C, Mutation, Blood

-PTNT / Prothrombin G20210A Mutation, Blood

-MTHFR / 5,10-Methylenetetrahydrofolate Reductase C677T, Mutation, Blood

-MTHP / 5,10-Methylenetetrahydrofolate Reductase C677T and A1298C Mutations, Blood


Specimen Minimum Volume

1 mL blood in a 3-mL ACD tube

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Ambient (preferred) 7 days
  Frozen  14 days
  Refrigerated  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Other Extracted DNA

Reference Values

Negative

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Day(s) and Time(s) Performed

Monday through Friday

Method Name

Direct Mutation Analysis

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81241-F5 (coagulation factor V) (eg, hereditary hypercoagulability) gene analysis, Leiden variant

 

LOINC Code Information

Test ID Test Order Name Order LOINC Value
F5DNA Factor V Leiden (R506Q) Mutation, B 21668-9

 

Result ID Test Result Name Result LOINC Value
21838 Factor V Leiden (R506Q) Mutation, B 21668-9
21839 F5DNA Interpretation 69049-5
21841 F5DNA Reviewed By 18771-6

Forms

1. New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available in Special Instructions:

-Informed Consent for Genetic Testing (T576)

-Informed Consent for Genetic Testing-Spanish (T826)

2. Coagulation Patient Information (T675) in Special Instructions

3. If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.