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HelpOrder Code MTRTI MatePair, Targeted Rearrangements, Oncology
Useful For
Second-tier testing in oncologic specimens when previous cytogenetic testing has detected an acquired chromosome abnormality of unknown significance
Determining the size, precise breakpoints, gene content, and any unappreciated complexity of abnormalities detected by other methods such as conventional chromosome and FISH studies
Providing important diagnostic, prognostic, and therapeutic information critical to proper patient management
Special Instructions
Method Name
Mate-Pair Whole Genome Sequencing
Reporting Name
MatePair, Targeted, OncologySpecimen Type
TissueShipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
1. Previous cytogenetic or FISH testing is required in order to perform this test. If previous testing was performed at another institution, supply a copy of those results. If sufficient information regarding the patient's known chromosome abnormality is not made available, this testing will be cancelled.
2. Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
3. Specimens that are submitted as cultured cells or cryo-frozen samples will need confirmation of abnormal karyotype with the specimen submitted to ensure that the abnormality is present before proceeding.
4. Please complete the Mate Pair Hematology/Oncology Patient Information sheet.
Specimen Required
Submit only 1 of the following specimens:
Supplies: Hank's Solution (T132)
Specimen Type: Tumor biopsy
Container/Tube: Sterile container with sterile Hank's balanced salt solution (T132), Ringer's solution, or normal saline
Specimen Volume: 0.5-3 cm(3) or larger
Supplies: Hank's Solution (T132)
Specimen Type: Lymph node
Container/Tube: Sterile container with sterile Hank's balanced salt solution (T132), Ringer's solution, or normal saline.
Specimen Volume: 1 cm(3)
Supplies: Hank's Solution (T132)
Specimen Type: Skin biopsy
Container/Tube: Sterile container with sterile Hank's balanced salt solution (T132), Ringer's solution, or normal saline.
Specimen Volume: 4-mm diameter
Collection Instructions:
1. Wash biopsy site with an antiseptic soap.
2. Thoroughly rinse area with sterile water.
3. Do not use alcohol or iodine preparations.
4. A local anesthetic may be used.
5. Biopsy specimens are best taken by punch biopsy to include full thickness of dermis.
Specimen Type: Snap Frozen tissue
Container/Tube: Polycon flat
Specimen Volume: 3 cm(3)/Lymph Node: 1 cm(3)/Skin Biopsy: 4 mm diameter
Specimen Type: Cultured tumor cells
Container/Tube: T-25 flask filled with transport media
Specimen Volume: T-25 flask
Specimen Type: Cryo-frozen cultured tumor cells
Container/Tube: Sterile container in media with DMSO or other safe cryopreservation solution
Specimen Volume: 5 million cells
Specimen Type: Cryo-Frozen tissue
Container/Tube: Polycon flat
Specimen Volume: 3 cm(3)/Lymph Node: 1 cm(3)/Skin Biopsy: 4-mm diameter
Specimen Minimum Volume
Tumor Biopsy: 3 cm(3)
Lymph Node: 1 cm(3)
Skin Biopsy: 4-mm diameter
Cultured tumor cells: T-25 flask
Cryo-frozen cultured tumor cells: 5 million cells
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Tissue | Varies | |
Reject Due To
No specimen should be rejected.
Reference Values
An interpretive report will be provided.
Day(s) and Time(s) Performed
Samples processed Monday through Friday. Results reported Monday through Friday, 8 a.m.-5 p.m.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
0013U
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MTRTI | MatePair, Targeted, Oncology | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 48023 | Result Summary | 50397-9 |
| 48326 | Result | In Process |
| 48024 | Nomenclature | 62356-1 |
| 48025 | Interpretation | 69965-2 |
| CG988 | Reason for Referral | 42349-1 |
| CG986 | Specimen | 31208-2 |
| 48028 | Source | 31208-2 |
| 48029 | Method | 49549-9 |
| 48030 | Additional Information | 48767-8 |
| 48032 | Released By | 18771-6 |
Forms
1. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (T576) is available in Special Instructions.
2. Mate Pair Hematology/Oncology Patient Information in Special Instructions
3. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Oncology Test Request (T729)