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Order Code EXHR Hematologic Disorders, DNA and RNA Extract and Hold, Varies

Useful For

Reserving nucleic acids on any specimen for which molecular analysis requiring DNA or RNA may be necessary at a future date, ensuring that adequate material for testing is available

Testing Algorithm

DNA and RNA will be extracted from the specimen and stored at -80° Celsius for one year from the date the extraction process was performed. A report of "Performed" will be sent and a $75.00 processing fee will be assessed. To request molecular testing from the Molecular Hematopathology Laboratory's test menu, call 800-533-1710. Additional charges will be assessed for testing performed.

 

For more information see Eosinophilia: Bone Marrow Diagnostic Algorithm.

Method Name

Nucleic Acid Extraction

Reporting Name

DNA and RNA Extract and Hold

Specimen Type

Varies


Shipping Instructions


Refrigerate specimens must arrive within 5 days of collection, and ambient specimens must arrive with 3 days (72 hours) of collection. Collect and package specimens as close to shipping time as possible.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 10 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

3. Label specimen as blood.

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send bone marrow specimen in original tube. Do not aliquot.

3. Label specimen as bone marrow.


Specimen Minimum Volume

Blood: 8 mL
Bone Marrow: 2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 5 days PURPLE OR PINK TOP/EDTA
  Ambient  72 hours PURPLE OR PINK TOP/EDTA

Reject Due To

Gross hemolysis Reject
Paraffin-embedded material
Fluids
Heparinized samples		 Reject

Reference Values

Not applicable

Method Description

DNA and RNA are extracted using automated systems. Both systems include a sample lysis step followed by binding of nucleic acid to a solid phase for separation from other cellular components and a final nucleic acid elution step.

Day(s) Performed

Monday through Saturday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.