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Order Code LAB1418 Vitamin A, Serum

Useful For

Diagnosing vitamin A deficiency and toxicity

 

Monitoring vitamin A therapy

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Vitamin A, S

Specimen Type

Serum


Specimen Required


Patient Preparation:

Fasting: 12 hours, required; infants should have specimen collected before next feeding.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.

Note: Serum must be removed from the red blood cells within 2 hours of collection.


Specimen Minimum Volume

0.25 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

0-6 years: 11.3-64.7 mcg/dL

7-12 years: 12.8-81.2 mcg/dL

13-17 years: 14.4-97.7 mcg/dL

≥18 years: 32.5-78.0 mcg/dL

Method Description

Deuterated vitamin A (d6-all-trans retinol) is added to serum as an internal standard. Vitamin A (all-trans retinol) and the deuterated internal standard are extracted from the specimens using on-line turbulent flow high-performance liquid chromagraphy and analyzed by liquid chromatography-tandem mass spectrometry using multiple reaction monitoring in positive mode.(Unpublished Mayo method)

Day(s) Performed

Sunday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84590

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VITA Vitamin A, S 2923-1

 

Result ID Test Result Name Result LOINC Value
7597 Vitamin A 2923-1

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.