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HelpOrder Code LAB1418 Vitamin A, Serum
Useful For
Diagnosing vitamin A deficiency and toxicity
Monitoring vitamin A therapy
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Vitamin A, SSpecimen Type
SerumSpecimen Required
Patient Preparation:
Fasting: 12 hours, required; infants should have specimen collected before next feeding.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.
Note: Serum must be removed from the red blood cells within 2 hours of collection.
Specimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 28 days |
| Ambient | 28 days | |
| Frozen | 28 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
0-6 years: 11.3-64.7 mcg/dL
7-12 years: 12.8-81.2 mcg/dL
13-17 years: 14.4-97.7 mcg/dL
≥18 years: 32.5-78.0 mcg/dL
Method Description
Deuterated vitamin A (d6-all-trans retinol) is added to serum as an internal standard. Vitamin A (all-trans retinol) and the deuterated internal standard are extracted from the specimens using on-line turbulent flow high-performance liquid chromagraphy and analyzed by liquid chromatography-tandem mass spectrometry using multiple reaction monitoring in positive mode.(Unpublished Mayo method)
Day(s) Performed
Sunday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84590
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.