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Order Code LAB1418 Vitamin A, Serum

Useful For

Diagnosing vitamin A deficiency or toxicity

 

Monitoring vitamin A therapy

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Vitamin A, S

Specimen Type

Serum


Specimen Required


Patient Preparation:

1. Fasting: 12 hours, required; infants should have specimen collected before next feeding

2. Patient must not consume any alcohol for 24 hours before specimen collection.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL serum

Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

Serum: 0.25 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

0-6 years: 11.3-64.7 mcg/dL

7-12 years: 12.8-81.2 mcg/dL

13-17 years: 14.4-97.7 mcg/dL

≥18 years: 32.5-78.0 mcg/dL

Method Description

Deuterated vitamin A (d6-all-trans retinol) is added to serum as an internal standard. Vitamin A (all-trans retinol) and the deuterated internal standard are extracted from the specimens and analyzed by liquid chromatography tandem mass spectrometry.(Unpublished Mayo method)

Day(s) Performed

Monday through Friday, Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84590

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VITA Vitamin A, S 2923-1

 

Result ID Test Result Name Result LOINC Value
7597 Vitamin A 2923-1

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.