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HelpOrder Code LAB1909 Erythropoietin, Serum
Additional Codes
| Mayo Test ID |
|---|
| EPO |
Reporting Name
Erythropoietin (EPO), SUseful For
An aid in distinguishing between primary and secondary polycythemia
Differentiating between appropriate secondary polycythemia (eg, high-altitude living, pulmonary disease, tobacco use) and inappropriate secondary polycythemia (eg, tumors)
Identifying candidates for erythropoietin (EPO) replacement therapy (eg, those with chronic renal failure)
Evaluating patients undergoing EPO replacement therapy who demonstrate an inadequate hematopoietic response
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.6 mL
Collection Instructions:
1. Morning collection, 7:30 a.m.-12 p.m. is preferred due to diurnal variation. For more information see Cautions.
2. Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 14 days | |
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross Icterus | OK |
Reference Values
2.6-18.5 mIU/mL
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Day(s) Performed
Monday through Friday
Method Name
Immunoenzymatic Assay
CPT Code Information
82668
Testing Algorithm
The following algorithms are available in Special Instructions:
-Erythrocytosis Evaluation Testing Algorithm
-Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation
-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood Evaluation
Special Instructions
Method Description
The Access erythropoietin (EPO) assay is a 2-site immunoenzymatic (sandwich) assay. A sample is added to a reaction vessel along with the paramagnetic particles coated with mouse monoclonal anti-EPO, blocking reagent and the alkaline phosphatase conjugate. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of EPO in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve.(Instruction manual: Beckman Coulter Access EPO. Beckman Coulter, Inc; 2019)
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)
-Benign Hematology Test Request (T755)