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HelpOrder Code LABGOR, MCLIP Phospholipid (Cardiolipin) Antibodies, IgM, Serum
Reporting Name
Phospholipid Ab IgM, SUseful For
The following clinical situations, when used in conjunction with other criterial antiphospholipid antibody tests:
-Unexplained arterial or venous thrombosis
-A history of pregnancy morbidity defined as 1 or more unexplained deaths of a morphologically normal fetus beyond the 10th week of gestation, 1 or more premature births before 34 weeks of gestation caused by severe preeclampsia or placental insufficiency, or 3 or more unexplained, consecutive spontaneous abortions before the 10th week of gestation with no identifiable maternal hormonal or anatomic, or maternal or paternal chromosomal causes
-Presence of a systemic autoimmune rheumatic disease, especially systemic lupus erythematosus
-Unexplained thrombocytopenia
-Presence of an unexplained cutaneous manifestations varying from livedo reticularis to cutaneous necrosis, such as leg ulcers
-Possible nonbacterial, thrombotic endocarditis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days |
Reference Values
MPL refers to IgM phospholipid units. One MPL unit is 1 microgram of IgM antibody.
Negative: <15.0 MPL)
Weakly positive: 15.0-39.9 MPL
Positive: 40.0-79.9 MPL
Strongly positive:≥80.0 MPL
Reference values apply to all ages.
CPT Code Information
86147
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat-treated specimen | Reject |
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Method Description
Purified cardiolipin antigen is bound to the wells of a polystyrene microwell plate under conditions that will preserve the antigen in its native state. Prediluted controls and diluted patient sera are added to separate wells, allowing any cardiolipin antibodies present to bind to the immobilized antigen. Unbound sample is washed away, and an enzyme-labeled antihuman IgM conjugate is added to each well. A second incubation allows the enzyme-labeled antihuman IgM to bind to any patient antibodies that have become attached to the microwells. After washing away any unbound enzyme-labeled antihuman IgM, the remaining enzyme activity is measured by adding a chromogenic substrate and measuring the intensity of the color that develops. After stopping the enzymatic production of colored product, the presence or absence of cardiolipin antibody is determined by comparing the sample optical density with that of a 5-point calibration curve. Results are reported out semiquantitatively in standard IgM anticardiolipin units.(Package inserts: QUANTA Lite ACA IgM III. Inova Diagnostics; Version 23, 08/2020)
Day(s) Performed
Monday through Saturday