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Order Code PBPFD Lead Profile Occupational Exposure, Blood


Ordering Guidance


This test is only for assessment of occupational exposure to lead. The preferred test for lead toxicity in children is blood lead. For more information see:

-PBDV / Lead, Venous, with Demographics, Blood

-PBDC / Lead, Capillary, with Demographics, Blood

 

The preferred screening test for suspicion of a hepatic porphyria is urine porphyrins. For more information see PQNRU / Porphyrins, Quantitative, Random, Urine.



Necessary Information


Include a list of medications the patient is currently taking.



Specimen Required


Both EDTA whole blood and heparin whole blood specimens are required.

 

Patient Preparation:

1. High concentrations of gadolinium and iodine are known to interfere with most inductively couple plasma mass spectrometry-based metal tests. If either gadolinium- or iodine-containing contrast media has been administered, a specimen should not be collected for 96 hours.

2. Patient should abstain from alcohol for 24 hours prior to specimen collection.

 

Specimen Type: Whole blood

Supplies:

-Metal Free B-D Tube (EDTA), 6 mL (T183)

-Metal Free B-D Tube (EDTA), 3 mL (T989)

-Microtainer (EDTA) Tube, 0.5 mL (T174)

Container/Tube:

Preferred: Royal blue-top BD Vacutainer Plus with EDTA blood collection tube (6 mL) (BD catalog no. 368381)

Acceptable: Royal blue-top BD vacutainer with EDTA blood collection tube (3 mL), BD Microtainer with EDTA, or royal blue-top Monoject trace element blood collection tube

Specimen Volume: 2 mL

Collection Instructions:

1. See Metal Analysis Specimen Collection and Transport for complete instructions.

2. Send whole blood specimen in original tube. Do not aliquot.

3. Refrigerate specimen as soon as possible.

 

Specimen Type: Whole blood

Container/Tube:

Preferred: Green top (sodium heparin)

Acceptable: Dark blue top (metal free heparin), green top (lithium heparin), or lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions: Refrigerate specimen as soon as possible after collection.


Useful For

Detecting lead toxicity due to occupational exposure

Profile Information

Test ID Reporting Name Available Separately Always Performed
PBB Lead, B Yes, (order PBDV) Yes
PPFE Protoporphyrins, Fractionation, WB Yes Yes
DEMO5 Patient Demographics No Yes

Method Name

PBB: Inductively Coupled Plasma Mass Spectrometry (ICP-MS)

PPFE: High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection

Reporting Name

Lead Profile Occ Exposure, B

Specimen Type

Whole blood

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Refrigerated 7 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Clotted blood Reject

Reference Values

LEAD: <3.5 mcg/dL

The Occupational Safety and Health Administration (OSHA) recommended limit for blood lead level is 40 mcg/dL (OSHA 1978).

The biological exposure index (BEI) for Pb in blood of exposed workers is 20 mcg/dL (ACGIH 2018).

 

Critical Values:

Pediatrics (≤15 years): ≥20.0 mcg/dL

Adults (≥16 years): ≥70.0 mcg/dL

 

PROTOPORPHYRINS, FRACTIONATION

Free Protoporphyrin: <20 mcg/dL

Zinc-Complexed Protoporphyrin: <60 mcg/dL

Method Description

Lead

The metal of interest is analyzed by inductively coupled plasma mass spectrometry.(Unpublished Mayo method)

 

Protoporphyrins, Fractionation

Extraction followed by fractionation by high-performance liquid chromatography. Zinc protoporphyrin and free protoporphyrin are separately quantitated.(Smith RM, Doran D, Mazur M, Bush B. High-performance liquid chromatographic determination of protoporphyrin and zinc protoporphyrin in blood. J Chromatogr.1980;181[3-4]:319-327; Gou EE, Balwani M, Bissell DM, et al. Pitfalls in erythrocyte protoporphyrin measurement for diagnosis and monitoring of protoporphyrias. Clin Chem. 2015;61[12]:1453-1456. doi:10.1373/clinchem.2015.245456)

Day(s) Performed

Monday, Wednesday, Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83655

82542