Home
HelpOrder Code PINS Proinsulin, Plasma
Reporting Name
Proinsulin, PUseful For
As part of the diagnostic workup of suspected insulinoma
As part of the diagnostic workup of patients with suspected prohormone convertase 1/3 deficiency
As part of the diagnostic workup of patients with suspected proinsulin variations
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Plasma EDTASpecimen Required
Patient Preparation:
1.Patient should fast for 8 hours before specimen collection.
2. Infants younger than 2 years should fast a maximum of 6 hours.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Ice-cooled, lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. After collection, place the whole blood on ice for at least 10 minutes, then centrifuge at refrigerated temperature.
2. Aliquot plasma into a plastic vial and send frozen.
Specimen Minimum Volume
0.25 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Frozen | 30 days |
Reference Values
3.6-22 pmol/L
Day(s) Performed
Tuesday, Friday
CPT Code Information
84206
Method Description
Sequential 2-site electrochemiluminescent immunoassay performed on the MesoScale Discovery instrument. The assay uses a monoclonal biotinylated anti-insulin capture antibody and an anti-C-peptide detection antibody labeled with SULFO-TAG. The signal is directly proportional to the amount of proinsulin in the sample. Assay calibration is traceable to the World Health Organization 1st International Standard for human proinsulin, NIBSC code: 09/296. This assay demonstrates no cross-reactivity with insulin or C-peptide.(Unpublished Mayo method)
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Method Name
Electrochemiluminescent Assay