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HelpOrder Code ADVQU Adenovirus DNA Detection and Quantification, Plasma
Ordering Guidance
Request this test only in at-risk individuals, such as organ or hematopoietic stem cell transplant recipients, suspected of developing disseminated adenoviral infection.
Shipping Instructions
1. Ship specimen frozen on dry ice only.
2. If shipment will be delayed for more than 24 hours, freeze plasma at -20 to -80° C (up to 30 days) until transport and ship on dry ice.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL (plasma)
Collection Instructions:
1. Centrifuge blood collection tube per manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).
2. Aliquot plasma into plastic vial.
Useful For
Aiding in the diagnosis of disseminated adenovirus infections in at-risk individuals
Measuring adenoviral load in plasma to monitor disease progression and antiviral response in individuals with disseminated infection
Method Name
Real-Time (Quantitative) Polymerase Chain Reaction (rt-qPCR)
Reporting Name
Adenovirus DNA Detect/Quant, PSpecimen Type
Plasma EDTASpecimen Minimum Volume
0.8 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma EDTA | Frozen (preferred) | 30 days |
| Refrigerated | 6 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | Reject |
| Gross icterus | OK |
Reference Values
Undetected
Method Description
This laboratory-developed, real-time, polymerase chain reaction (PCR) assay is performed on the cobas 5800 system. It amplifies and detects a 53-base pair (bp) sequence (77-bp long when including the 5' flap on the PCR primers) within the adenoviral hexon protein-encoding gene and uses an MGB-TaqMan hydrolysis probe labeled with FAM fluorophore (emission wavelength of 521 nm) for real-time detection of the amplified adenoviral sequences. The cobas 5800 assay-specific Utility Channel Analysis Package program analyzes the amplified target fluorescence signal to determine positivity and adenovirus DNA concentrations in individual PCR. The assay is calibrated to the First World Health Organization International Standard for human adenovirus for nucleic acid amplification techniques (NIBSC code: 16/234; adenovirus species C, type 2), and results are reported in IU/mL.(Package insert: cobas omni Utility Channel Reagent Kit for use on the cobas 5800/6800/8800 Systems. Roche Molecular Systems, Inc.; Doc. Rev. 3.0; 11/2022)
Day(s) Performed
Varies
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87799
Forms
If not ordering electronically, complete, print, and send an Microbiology Test Request (T244) with the specimen.