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Order Code BAP Bone Alkaline Phosphatase, Serum

Additional Codes

Mayo Test ID
BAP

Reporting Name

Bone Alkaline Phosphatase, S

Useful For

Diagnosis and assessment of severity of metabolic bone disease including Paget disease, osteomalacia, and other states of high bone turnover

 

Monitoring efficacy of antiresorptive therapies including postmenopausal osteoporosis treatment

 

The assay is not intended as a screening test for osteoporosis.

 

Measurements of bone turnover markers are not useful for the diagnosis of osteoporosis; diagnosis of osteoporosis should be made based on bone density.

Specimen Type

Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  90 days
  Ambient  7 days

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Reference Values

Males

<2 years: 25-221 mcg/L

2-9 years: 27-148 mcg/L

10-13 years: 35-169 mcg/L

14-17 years: 13-111 mcg/L

Adults: ≤20 mcg/L

 

Females

<2 years: 28-187 mcg/L

2-9 years: 31-152 mcg/L

10-13 years: 19-177 mcg/L

14-17 years: 7-41 mcg/L

Adults

Premenopausal: ≤14 mcg/L

Postmenopausal: ≤22 mcg/L

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Day(s) Performed

Monday through Saturday

Method Name

Immunoenzymatic Assay

CPT Code Information

84080

Method Description

The instrument used is a Beckman Coulter Unicel DXI 800. The Access Ostase assay is a one-step immunoenzymatic assay used to measure bone alkaline phosphatase (BAP) in human serum. The assay utilizes a mouse monoclonal antibody specific to BAP and paramagnetic particles coated with goat antimouse antibodies. BAP in the patient's specimen binds to the anti-BAP mouse antibody, which in turn is captured by the solid phase antimouse antibody. After washing to remove any unbound material, a chemiluminescent substrate is added to the reaction vessel. The BAP present acts on the substrate to produce light, which is measured with a luminometer. The amount of light produced is directly proportional to the amount of BAP in the specimen. The amount of analyte in the specimen is determined from a stored, multipoint calibration curve.(Package insert: Access Ostase. Beckman-Coulter; 2019)