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HelpOrder Code BORDG Bordetella pertussis Antibody, IgG, Serum
Useful For
Diagnosis of recent infection with Bordetella pertussis in patients with symptoms consistent with whooping cough for 2 or more weeks
This test should not be used in neonates, young infants or in children between the ages of 4 to 7 years as the routine childhood vaccine schedule may interfere with result interpretation.
This test should not be used as a test of cure, to monitor response to treatment, or to determine vaccine status.
Method Name
Enzyme-Linked Immunosorbent Assay (ELISA)
Reporting Name
B. pertussis Ab, IgG, SSpecimen Type
SerumOrdering Guidance
This test should be ordered in patients with 2 or more weeks of symptoms consistent with whooping cough. Nucleic acid amplification testing for Bordetella pertussis should be used in patients who have been symptomatic less than 2 weeks; order BPRP / Bordetella pertussis and Bordetella parapertussis, Molecular Detection, PCR, Varies.
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 30 days |
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
| Heat inactivated | Reject |
Reference Values
≥100 IU/mL (Positive)
40-<100 IU/mL (Borderline)
<40 IU/mL (Negative)
Reference values apply to all ages.
Method Description
The antipertussis toxin (PT) IgG enzyme-linked immunosorbent assay (ELISA) test is a quantitative assay. Microtiter wells are coated with PT from Bordetella pertussis and diluted patient samples, calibrators, and controls are incubated in the wells. If present, antibodies to Bordetella pertussis will bind to the antigen. After wells are washed, enzyme-labeled antihuman IgG is added, and wells are incubated a second time. After incubation, wells are washed and a tetramethylbenzidine chromogen/substrate solution is added and wells are incubated. Stop solution is added to stop the reaction. Wells are read using a microplate reader with 450/620 nm wavelength. Calibrator values are plotted to make a point-to-point standard curve. Sample antibody concentrations are determined using the standard curve.(Package insert: Anti-Bordetella pertussis toxin ELISA (IgG) Test Instructions. EUROIMMUN US; 03/05/2019)
Day(s) Performed
Thursday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86615
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.