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Order Code CARNP Carbapenemase Detection-Carba NP Test, Varies

Infectious

Useful For

Confirming carbapenemase production from pure isolates of Enterobacterales or Pseudomonas aeruginosa

Additional Tests

Test ID Reporting Name Available Separately Always Performed
CARNB Carbapenemase-Carba NP Test No Yes

Reporting Name

Carbapenemase-Carba NP Test

Specimen Type

Varies


Shipping Instructions


1. For shipping information see Infectious Specimen Shipping Guidelines.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.



Necessary Information


Specimen source and organism identification are required.



Specimen Required


Specimen Type: Organism

Supplies: Infectious Container, Large (T146)

Container/Tube: Slant

Specimen Volume: Isolate

Collection Instructions: Submit Enterobacterales or Pseudomonas aeruginosa isolate in pure culture (ie, not mixed with other organisms), actively growing.


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reject Due To

Agar plate Reject

Reference Values

Negative

Method Description

A pure bacterial isolate is emulsified into cell lysis buffer in 2 tubes: one contains the base indicator solution (phenol red with zinc salts) alone and the other contains the base indicator solution plus imipenem (6 mg/mL). The tubes are incubated at 37° C for 2 hours. A positive reaction is indicated by a color change from red to yellow as a result of hydrolysis of the beta-lactam ring of imipenem.(Vasoo S, Cunningham SA, Kohner PC, et al: Comparison of a novel, rapid chromogenic biochemical assay, the Carba NP test, with the modified Hodge test for detection of carbapenemase-producing Gram-negative bacilli. J Clin Microbiol. 2013;51[9]:3097-3101)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Colorimetric Detection of Carbapenem Hydrolysis

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Specimen Minimum Volume

See Specimen Required