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HelpOrder Code CARNP Carbapenemase Detection-Carba NP Test, Varies
Infectious
Useful For
Confirming carbapenemase production from pure isolates of Enterobacterales or Pseudomonas aeruginosa
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CARNB | Carbapenemase-Carba NP Test | No | Yes |
Special Instructions
Reporting Name
Carbapenemase-Carba NP TestSpecimen Type
VariesShipping Instructions
1. For shipping information see Infectious Specimen Shipping Guidelines.
2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.
Necessary Information
Specimen source and organism identification are required.
Specimen Required
Preferred:
Specimen Type: Pure culture of Enterobacterales or Pseudomonas aeruginosa from source cultured
Supplies: Infectious Container, Large (T146)
Container/Tube: Agar slant or other appropriate media
Collection Instructions:
1. Perform isolation of bacterial isolate.
2. Submit Enterobacterales or Pseudomonas aeruginosa isolate in pure culture actively growing. Do not submit mixed cultures.
3. Place the slant into the secondary infectious container for shipment.
4. Each isolate must be submitted under a separate order.
Acceptable:
Specimen Type: Enterobacterales or Pseudomonas aeruginosa isolate swab
Supplies:
-E-Swab (T853)
-Infectious Container, Large (T146)
Container/Tube: E-Swab collection and transport system
Collection Instructions:
1. Perform isolation of bacterial isolate.
2. Utilize the E-swab to obtain an adequate sample of Enterobacterales or Pseudomonas aeruginosa isolate in pure culture. Do not submit mixed cultures.
3. Place swab into the transport system containing 1-mL liquid Amies transport medium.
4. If needed, break off end of swab and close the transport tube.
5. Place the transport system into the secondary infectious container for shipment.
6. Each isolate must be submitted under a separate order.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
| Agar plate | Reject |
Reference Values
Negative
Method Description
A pure bacterial isolate is emulsified into cell lysis buffer in 2 tubes: one contains the base indicator solution (phenol red with zinc salts) alone and the other contains the base indicator solution plus imipenem (6 mg/mL). The tubes are incubated at 37° C for 2 hours. A positive reaction is indicated by a color change from red to yellow as a result of hydrolysis of the beta-lactam ring of imipenem.(Vasoo S, Cunningham SA, Kohner PC, et al. Comparison of a novel, rapid chromogenic biochemical assay, the Carba NP test, with the modified Hodge test for detection of carbapenemase-producing Gram-negative bacilli. J Clin Microbiol. 2013;51[9]:3097-3101)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CARNP | Carbapenemase-Carba NP Test | 74676-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CARNP | Carbapenemase-Carba NP Test | 74676-8 |
Method Name
Colorimetric Detection of Carbapenem Hydrolysis
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Specimen Minimum Volume
See Specimen Required
CPT Code Information
87182