Home
HelpOrder Code CARU Cyclic Adenosine Monophosphate (cAMP), Urinary Excretion, Serum and Urine
Specimen Required
Both serum and urine are required. Serum must be obtained at the time of the urine collection.
Specimen Type: Serum
Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Serum gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged, and the serum aliquoted into a plastic vial within 2 hours of collection.
3. Label specimen as serum.
Specimen Type: Urine
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Container/Tube: Plastic vial
Specimen Volume: 5 mL
Collection Instructions:
1. Collect a random urine specimen.
2. Label specimen as urine.
Useful For
Differential diagnosis of hypercalcemia
As an adjunct to serum parathyroid hormone measurements, especially in the diagnosis of parathyroid hormone resistance states, such as pseudohypoparathyroidism
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ACREA | Creatinine, S | Yes, (order CRTS1) | Yes |
| CAMP | Cyclic Amp, Urinary Excretion | No | Yes |
| CRETR | Creatinine, Random, U | Yes, (order RCTUR) | Yes |
Method Name
ACREA, CRETR: Enzymatic Colorimetric Assay
CAMP: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Cyclic Amp, Urinary ExcretionSpecimen Type
SerumUrine
Specimen Minimum Volume
Serum: 0.5 mL
Urine: 2.0 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 7 days | |
| Frozen | 90 days | ||
| Urine | Refrigerated (preferred) | 28 days | |
| Frozen | 28 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
CYCLIC AMP
1.3-3.7 nmol/dL of glomerular filtrate
CREATININE, SERUM
Males
0-11 months: 0.17-0.42 mg/dL
1-5 years: 0.19-0.49 mg/dL
6-10 years: 0.26-0.61 mg/dL
11-14 years: 0.35-0.86 mg/dL
≥15 years: 0.74-1.35 mg/dL
Females
0-11 months: 0.17-0.42 mg/dL
1-5 years: 0.19-0.49 mg/dL
6-10 years: 0.26-0.61 mg/dL
11-15 years: 0.35-0.86 mg/dL
≥16 years: 0.59-1.04 mg/dL
CREATININE, URINE
No reference values apply. Interpret with other clinical data.
Method Description
Adenosine 3',5'-cyclic monophosphate (cAMP) is isolated from the urine using a single anion exchange column. An internal standard (8-methyl amino cAMP) is used to correct for recovery losses. Once the cAMP has been eluted from the column, it is added to a solution and injected onto the liquid chromatography tandem mass spectrometry system. Quantitation is by peak area measurement against a calibration standard containing known quantities of cAMP and internal standard. Urine and serum creatinine levels are used to determine the clearance of cAMP from the kidneys.(Unpublished Mayo method)
Creatinine:
The enzymatic method is based on the determination of sarcosine from creatinine with the aid of creatininase, creatinase, and sarcosine oxidase. The liberated hydrogen peroxide is measured via a modified Trinder reaction using a colorimetric indicator. Optimization of the buffer system and the colorimetric indicator enables the creatinine concentration to be quantified both precisely and specifically.(Package insert: Creatinine plus v2. Roche Diagnostics; V15.0, 03/2019)
Day(s) Performed
Wednesday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82030
82570
82565