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Order Code CD4RT CD4 T-Cell Recent Thymic Emigrants, Blood

Reporting Name

CD4 RTE, Flow Cytometry

Useful For

Evaluating thymic reconstitution in patients following hematopoietic cell transplantation, chemotherapy, immunomodulatory therapy, and immunosuppression

 

Evaluating thymic recovery in patients who are HIV-positive and on highly active antiretroviral therapy

 

Evaluating thymic output in patients with DiGeorge syndrome or other cellular immunodeficiencies

 

Assessing the naive T-cell compartment in a variety of immunological contexts (autoimmunity, cancer, immunodeficiency, and transplantation)

 

Identification of thymic remnants post-thymectomy for malignant thymoma or as an indicator of relapse of disease (malignant thymoma) or other contexts of thymectomy

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole Blood EDTA


Shipping Instructions


Testing is performed Monday through Friday. Specimens not received by 4 p.m. (CST) on Friday may be canceled.

 

Samples arriving on the weekend and observed holidays may be canceled.

 

Collect and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the box.

 

It is recommended that specimens arrive within 24 hours of collection.



Necessary Information


Ordering healthcare professional name and phone number are required.



Specimen Required


Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

Container/Tube: Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Specimen Minimum Volume

1.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient 48 hours PURPLE OR PINK TOP/EDTA

Reference Values

CD4 Absolute

Males

1 month-17 years: 153-1745 cells/mcL

18-70 years: 290-1,175 cells/mcL

Reference values have not been established for patients that are younger than 30 days of age.

Reference values have not been established for patients that are older than 70 years of age.

 

Females

1 month-17 years: 582-1630 cells/mcL

18-70 years: 457-1,766 cells/mcL

Reference values have not been established for patients that are younger than 30 days of age.

Reference values have not been established for patients that are older than 70 years of age.

 

CD4 RTE %

Males

1 month-17 years: 19.4-60.9%

18-25 years: 6.4-51.0%

26-55 years: 6.4-41.7%

≥56 years: 6.4-27.7%

Reference values have not been established for patients that are younger than 30 days of age.

Reference values have not been established for patients that are older than 70 years of age.

 

Females

1 month-17 years: 25.8-68.0%

18-25 years: 6.4-51.0%

26-55 years: 6.4-41.7%

≥56 years: 6.4-27.7%

Reference values have not been established for patients that are younger than 30 days of age.

Reference values have not been established for patients that are older than 70 years of age.

 

CD4 RTE Absolute

Males

1 month-17 years: 50.0-926.0 cells/mcL

18-70 years: 42.0-399.0 cells/mcL

Reference values have not been established for patients that are younger than 30 days of age.

Reference values have not been established for patients that are older than 70 years of age.

 

Females

1 month-17 years: 170.0-1007.0 cells/mcL

18-70 years: 42.0-832.0 cells/mcL

Reference values have not been established for patients that are younger than 30 days of age.

Reference values have not been established for patients that are older than 70 years of age.

Day(s) Performed

Monday through Friday

CPT Code Information

86356

Method Description

CD4 recent thymic emigrants are assessed in peripheral blood drawn in EDTA tubes. A panel of antibodies is used for the assay: CD3, CD4, CD31, CD45RA, and CD45RO, conjugated to various fluorochromes. The blood is incubated with the antibodies in the dark, followed by red blood cell lysis. Absolute counts are obtained using BD TruCount tubes. The sample is then centrifuged and resuspended in a paraformaldehyde solution for analysis on a flow cytometer.(Unpublished Mayo method)

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Method Name

Flow Cytometry