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Order Code CEAPT Carcinoembryonic Antigen (CEA), Peritoneal Fluid

Reporting Name

CEA, Peritoneal Fluid

Useful For

An adjunct to cytology to differentiate between malignancy-related and benign causes of ascites formation

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Peritoneal


Specimen Required


Sources: Peritoneal, abdominal, ascites, paracentesis fluid (peritoneal washings are not acceptable)

Container/Tube: Plain, plastic, screw top tube

Specimen Volume: 2 mL


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Peritoneal Frozen (preferred) 90 days
  Ambient  7 days
  Refrigerated  7 days

Reference Values

An interpretive report will be provided.

Day(s) Performed

Monday through Saturday

CPT Code Information

82378

Method Description

The instrument used is Beckman Coulter UniCel DXI 800. The Access CEA assay is a 2-site immunoenzymatic sandwich assay using mouse monoclonal carcinoembryonic antigen (CEA) antibodies that react with different epitopes of CEA. A sample is added to a reaction vessel, along with the first CEA monoclonal antibodies-alkaline phosphatase conjugate and the second CEA monoclonal antibodies bound to paramagnetic particles. The incubation is followed by a magnetic separation and washing. A chemiluminescent substrate is added to the vessel, and the light generated by the reaction is measured with a luminometer. The light production is proportional to the concentration of CEA in the sample. The amount of analyte in the sample is determined by means of a stored, multipoint calibrator curve.(Package insert: Access CEA Assay, Beckman Coulter, Inc; 2024)

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Method Name

Immunoenzymatic Assay

Forms

If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.