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HelpOrder Code CMVLR Cytomegalovirus (CMV) Molecular Detection, PCR, Lower Respiratory
Ordering Guidance
For plasma specimens, order CMVQN / Cytomegalovirus (CMV) DNA Detection and Quantification by Real-Time PCR, Plasma.
Necessary Information
Specimen source is required.
Specimen Required
Specimen Type: Lower respiratory
Source: Bronchial washing, bronchoalveolar lavage, fluid/washings from lung, sputum, tracheal secretions, tracheal aspirates
Container/Tube:
Preferred: Sterile, screwcap, 5-mL aliquot tube
Acceptable: Sterile container
Specimen Volume: 1 mL
Collection Instructions: Do not centrifuge.
Useful For
Rapid qualitative detection of cytomegalovirus (CMV) DNA in lower respiratory specimens
This test is not intended for the monitoring of CMV disease progression or response to therapy.
Method Name
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reporting Name
Cytomegalovirus, PCR, Lower RespSpecimen Type
VariesSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Frozen | 7 days |
Reject Due To
| Lower respiratory swab Calcium alginate-tipped swab Wood swab Transport swab containing gel Feces Paraffin blocks Tissue specimens Tissue biopsy Bronchial brushings Heat-inactivated specimens Lower respiratory in transport media |
Reject |
Reference Values
Negative
Reference values apply to all ages.
Method Description
The Simplexa Congenital CMV (cytomegalovirus) Direct assay is a real-time polymerase chain reaction (PCR) system that enables the direct amplification and detection of CMV DNA from lower respiratory specimens without nucleic acid extraction. The system consists of the Simplexa Congenital CMV Direct Reaction Mix, the LIAISON MDX (with LIAISON MDX Studio Software), the direct amplification disc, and associated accessories.
In the Simplexa Congenital CMV Direct assay, bifunctional fluorescent probe-primers are used together with corresponding reverse primers to amplify CMV DNA. A well-conserved region of the CMV UL83 gene is targeted to identify CMV DNA. An internal control is used to detect PCR failure or inhibition.(Package insert: Simplexa Congenital CMV Direct. Diasorin; REF MOL2255. Rev. 01, 11/2022)
Day(s) Performed
Monday through Sunday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87496