Order Code CTP0 Catecholamine Fractionation, Supine, Plasma, Free
Specimen Required
Only orderable as part of profile. For more information see CATPA / Catecholamine, Endocrine Study, Plasma.
Patient Preparation:
1. Discontinue drugs that release epinephrine, norepinephrine, or dopamine, or hinder their metabolism, for at least 1 week before obtaining the specimen (see Cautions for details). If this is not possible for medical reasons, contact the laboratory and discuss whether a shorter drug-withdrawal period may be possible in a particular case.
2. Unless the purpose of the measurement is drug monitoring, discontinue any epinephrine, norepinephrine, or dopamine injections/infusions for at least 12 hours before specimen draw.
3. The patient must refrain from eating, using tobacco, and drinking caffeinated beverages for at least 4 hours before the specimen is collected.
Supplies: Catecholamine tubes containing EDTA-sodium metabisulfite solution (T066) (tubes have a 6-month. expiration time)
Collection Container/Tube:
Preferred: 10-mL Catecholamine tubes containing EDTA-sodium metabisulfite solution
Acceptable: 6-mL Catecholamine tubes containing EDTA-sodium metabisulfite solution
Submission Container/Tube: Plastic vial
Specimen Volume: 3 mL per collection
Collection Instructions:
1. Drawing from a catheter is required.
2. Calm the patient by giving complete instructions and reassurance regarding the procedure.
3. Insert an indwelling intravenous catheter. Flush with 3 mL of saline, using positive pressure.
4. Have the patient rest for 30 minutes in the supine position in a quiet room.
5. At the end of the 30 minutes, withdraw and discard a minimum of 3 mL of blood to remove the saline out of the catheter.
6. If provocative sampling (eg, standing specimen) is required, perform provocative maneuver immediately after obtaining supine specimen. Obtain standing specimen immediately.
7. For each specimen, draw blood into the chilled EDTA-sodium metabisulfite 10-mL tube.
8. Specimen must remain at refrigerated temperature during processing and transport.
9. Separate plasma in a refrigerated centrifuge within 30 minutes of draw.
10. Freeze specimen immediately.
Useful For
Diagnosis of pheochromocytoma and paraganglioma in specimens collected from individuals in a supine position, as an auxiliary test to fractionated plasma and urine metanephrine measurements
Diagnosis and follow-up of patients with neuroblastoma and related tumors, as an auxiliary test to urine vanillylmandelic acid and homovanillic acid measurements
Evaluation of patients with autonomic dysfunction/failure or autonomic neuropathy
Method Name
Only orderable as part of profile. For more information see CATPA / Catecholamine, Endocrine Study, Plasma.
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Catecholamine, Fract, Supine, PSpecimen Type
Plasma EDTA MetaSpecimen Minimum Volume
4 mL
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Plasma EDTA Meta | Frozen | 28 days |
Reject Due To
Gross hemolysis | Reject |
Gross lipemia | OK |
Gross icterus | OK |
Reference Values
Only orderable as part of a profile. For more information see CATPA / Catecholamine, Endocrine Study, Plasma.
Method Description
Catecholamines are adsorbed onto activated alumina at pH 8.6, washed, and eluted. The eluate is derivatized and then analyzed for norepinephrine, epinephrine, and dopamine using tandem mass spectrometry.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Performing Laboratory

CPT Code Information
82384