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HelpOrder Code EGFRS EGFR Gene, Targeted Mutation Analysis, 51 Mutation Panel, Tumor
Necessary Information
Pathology report must accompany specimen for testing to be performed.
Specimen Required
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Tissue, smear stained with Diff Quik, fine needle aspirate (FNA), or pleural fluid in cell blocks
Container/Tube: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tissue block.
Acceptable:
Specimen Type: Tissue, smear stained with Diff Quik, FNA, or pleural fluid in cell block or cytology blood smear
Container/Tube: Slides
Specimen Volume: 1 Hematoxylin and eosin stained and 5 unstained
Collection Instructions:
1. For FFPE tissue, FNA or pleural fluid: Submit 1 slide stained with hematoxylin and eosin and 5 unstained, non-baked slides with 5 to 10-micron thick sections of the tumor tissue.
2. For cytology blood smear: Submit up to 2 slides stained with Diff Quik
Useful For
Identifying non-small cell lung cancers that may respond to epidermal growth factor receptor-targeted therapies
Additional Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SLIRV | Slide Review in MG | No, (Bill Only) | Yes |
Testing Algorithm
When this test is ordered, slide review will always be performed at an additional charge.
Method Name
Polymerase Chain Reaction (PCR)
Reporting Name
EGFR Gene, Mutation Analysis, TumorSpecimen Type
VariesSpecimen Minimum Volume
Formalin-fixed, paraffin-embedded tissue block or Slides: see Specimen Required
Cytology smear stained with Diff Quik: >1000 cells
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
| Specimens that have been decalcified (all methods) | Reject |
Reference Values
An interpretive report will be provided.
Method Description
All ordered specimens will undergo EGFR testing. The EGFR test is a qualitative polymerase chain reaction (PCR)-based assay employing fluorescently labeled probes that are used to detect exon 18 (G719A/C/S), exon 21 (L858R, L861Q), exon 20 (T790M, S768I) mutations, exon 19 deletions and exon 20 insertions of the EGFR gene.(Unpublished Mayo method)
|
Exon |
Mutation |
Protein change |
Nucleotide change |
Genotype |
|
18 |
G719A |
p.Gly719Ala |
c.2156G>C |
G719A/C/S |
|
G719C |
p.Gly719Cys |
c.2155G>T |
||
|
G719C |
p.Gly719Cys |
c.2154_2155delinsTT |
||
|
G719S |
p.Gly719Ser |
c.2155G>A |
||
|
19 |
Deletion 9 |
p.Leu747_Ala750delinsPro |
c.2238_2248delinsGC |
Exon 19 deletion |
|
c.2239_2248delinsC |
||||
|
p.Leu747_Ala750delinsSer |
c.2240_2248del |
|||
|
p.Leu747_Glu749del |
c.2239_2247del |
|||
|
Deletion 12 |
p.Leu747_Thr751delinsPro |
c.2239_2251delinsC |
||
|
p.Leu747_Thr751delinsSer |
c.2240_2251del |
|||
|
Deletion 15 |
p.Glu746_Ala750del |
c.2235_2249del |
||
|
c.2236_2250del |
||||
|
p.Leu747_Thr751del |
c.2239_2253del |
|||
|
c.2240_2254del |
||||
|
c.2238_2252del |
||||
|
p.Glu746_Thr751delinsAla |
c.2237_2251del |
|||
|
p.Glu746_Thr751delinsIle |
c.2235_2252delinsAAT |
|||
|
p.Glu746_Thr751delinsVal |
c.2237_2252delinsT |
|||
|
p.Lys745_Ala750delinsThr |
c.2234_2248del |
|||
|
p.Glu746_Thr751delinsLeu |
c.2236_2253delinsCTA |
|||
|
p.Glu746_Thr751delinsVal |
c.2237_2253delinsTA |
|||
|
p.Glu746_Thr751delinsAla |
c.2235_2251delinsAG |
|||
|
p.Glu746_Thr751delinsGln |
c.2236_2253delinsCAA |
|||
|
p.Ile744_Ala750delinsValLys |
c.2230_2249delinsGTCAA |
|||
|
Deletion 18 |
p.Leu747_Pro753delinsSer |
c.2240_2257del |
||
|
p.Glu746_Ser752delinsVal |
c.2237_2255delinsT |
|||
|
p.Leu747_Ser752del |
c.2239_2256del |
|||
|
p.Glu746_Thr751del |
c.2236_2253del |
|||
|
p.Leu747_Pro753delinsGln |
c.2239_2258delinsCA |
|||
|
p.Glu746_Ser752delinsAla |
c.2237_2254del |
|||
|
p.Glu746_Ser752delinsAsp |
c.2238_2255del |
|||
|
p.Glu746_Pro753delinsValSer |
c.2237_2257delinsTCT |
|||
|
p.Glu746_Ser752delinsIle |
c.2236_2255delinsAT |
|||
|
c.2236_2256delinsATC |
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|
p.Glu746_Ser752delinsVal |
c.2237_2256delinsTT |
|||
|
c.2237_2256delinsTC |
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|
c.2235_2255delinsGGT |
||||
|
Deletion 21 |
p.Leu747_Pro753del |
c.2238_2258del |
||
|
p.Glu746_Ser752del |
c.2236_2256del |
|||
|
Deletion 24 |
p.Ser752_Ile759del |
c.2253_2276del |
||
|
20 |
T790M |
p.Thr790Met |
c.2369C>T |
T790M |
|
S768I** |
p.Ser768Ile |
c.2303G>T |
S768I |
|
|
InsG |
p.Asp770_Asn771insGly |
c.2310_2311insGGT |
Exon 20 insertion |
|
|
InsASV(9) |
p.Val769_Asp770insAlaSerVal |
c.2307_2308insGCCAGCGTG |
||
|
InsASV(11) |
p.Val769_Asp770insAlaSerVal |
c.2309_2310delinsCCAGCGTGGAT |
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|
InsSVD |
p.Asp770_Asn771insSerValAsp |
c.2311_2312insGCGTGGACA |
||
|
InsH |
p.His773_Val774insHis |
c.2319_2320insCAC |
||
|
21 |
L858R |
p.Leu858Arg |
c.2573T>G |
L858R |
|
c.2573_2574delinsGT |
||||
|
c.2573_2574delinsGA |
||||
|
L861Q |
p.Leu861Gln |
c.2582T>A |
L861Q |
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81235-EGFR (epidermal growth factor receptor) (eg, non-small cell lung cancer) gene analysis, common variants (eg, exon 19 LREA deletion, L858R, T790M, G719A, G719S, L861Q)
88381-Microdissection, manual
Forms
If not ordering electronically, complete, print, and send an Oncology Test Request (T729) with the specimen.