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Order Code EURO Phospholipase A2 Receptor, Enzyme Linked Immunosorbent Assay, Serum

Method Name

Only orderable as part of a profile. For more information see PMND1 / Primary Membranous Nephropathy Diagnostic Cascade, Serum

 

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Phospholipase A2 Receptor, ELISA, S

Specimen Type

Serum


Specimen Required


Only orderable as part of a profile. For more information see PMND1 / Primary Membranous Nephropathy Diagnostic Cascade, Serum.

 

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial within 2 hours of collection


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days
  Ambient  8 hours

Reference Values

Only orderable as part of a profile. For more information see PMND1 / Primary Membranous Nephropathy Diagnostic Cascade, Serum.

 

<14 RU/mL: Negative

14 to 19 RU/mL: Borderline

≥20 RU/mL: Positive

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83520

Useful For

Distinguishing primary from secondary membranous nephropathy

Reject Due To

Gross hemolysis Reject
Gross lipemia OK
Gross icterus OK

Method Description

The test kit provides microtiter strips, each with 8 break-off reagent wells. In the case of positive samples, specific IgG antibodies (also IgA and IgM) will bind to the antigens. To detect the bound antibodies, a second incubation is carried out using an enzyme-labelled antihuman IgG (enzyme conjugate) catalyzing a color reaction.(Package insert: Anti-PLA2R ELISA [IgG] Kit, EUROIMMUN US; V 07/08/2020)

Day(s) Performed

Monday, Wednesday, Friday