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HelpOrder Code KINM Ki-67(MIB-1), Gastrointestinal/Pancreatic Neuroendocrine Tumors, Quantitative Immunohistochemistry, Manual
Ordering Guidance
This is not an orderable test. If ordering for diagnostic purposes, order PATHC / Pathology Consultation and request the stain.
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) to the outside of the transport container before putting into the courier mailer.
Necessary Information
1. Pathologist's name, address, and phone number are required.
2. Include accompanying pathology report stating the final diagnosis. If not available, a preliminary diagnosis is acceptable.
Specimen Required
This is not an orderable test. Order PATHC / Pathology Consultation. The consultant will determine the need for special stains.
Supplies: Pathology Packaging Kit (T554)
Specimen Type:
Preferred: Formalin-fixed, paraffin-embedded tissue block containing neuroendocrine tumor of the pancreas or gastrointestinal (GI) tract including metastases.
Acceptable: 2 unstained sections, containing neuroendocrine tumor of the pancreas or GI tract including metastases, on charged slides cut at 4 microns <1 month ago. Tissue on the slides should have been fixed in 10% neutral buffered formalin.
Submission Container/Tube: Pathology Packaging Kit
Collection Instructions: Submit formalin-fixed, paraffin-embedded tissue block
Additional Information: Paraffin block will be returned with the final report.
Useful For
Determining proliferation of tumor cells in paraffin-embedded tissue blocks from patients diagnosed with neuroendocrine tumors of the pancreas or gastrointestinal tract including metastases
Testing Algorithm
Includes pathology consultation charged separately.
Method Name
This is not an orderable test. Order PATHC / Pathology Consultation. The consultant will determine the need for special stains.
Immunohistochemistry, Semi- Quantitation, Hot-Spot Technique
Reporting Name
Ki67 GI/Pancreas NET IHC ManualSpecimen Type
SpecialSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Special | Ambient (preferred) | ||
| Refrigerated | |||
Reject Due To
No specimen should be rejected.Reference Values
This is not an orderable test. Order PATHC / Pathology Consultation. The consultant will determine the need for special stains.
Varies by tumor type; values reported from 0% to 100%
Method Description
A 4-micron thick section is cut from the paraffin block. The section is stained with an immunoperoxidase method using the monoclonal antibody Ki-67 (MIB-1 clone). This is the paraffin nuclear epitope to the Ki-67 antigen. Any nucleus that has an antigen-antibody complex will cause the bright-field, brown chromogen, diaminobenzidine (DAB), to precipitate onto it. All nuclei, both DAB-positive and -negative, are counterstained with diluted hematoxylin.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
88360