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Order Code KRASW KRAS Somatic Mutation Analysis, Peritoneal Fluid


Shipping Instructions


Send specimens refrigerated (4° C)



Necessary Information


1. A pathology report (final or preliminary) is required and must accompany specimen for testing to be performed.

2. The following information must be included in the report provided.

-Patient name and second identifier

-Date of fluid collection

-Source of the fluid



Specimen Required


Container/Tube: 50-mL Falcon tube

Preferred: Fresh, peritoneal washing; no fixatives added to wash

Specimen Volume: Two 50-mL Falcon tubes

Collection Instructions: Containers must be labeled with two unique patient identifiers.


Useful For

Staging of the pancreatic ductal adenocarcinoma(1)

Method Name

Droplet Digital Polymerase Chain Reaction (ddPCR)

Reporting Name

KRAS Mutation Analysis, Peritoneal

Specimen Type

Varies

Specimen Minimum Volume

100 mL of peritoneal washing

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated

Reject Due To

Fixative added Reject

Reference Values

An interpretive report will be provided.

Method Description

Droplet digital polymerase chain reaction is used to test for the presence of KRAS codon 12, 13, 61, and 146 mutations.(Yonkus JA, Alva-Ruiz R, Abdelrahman AM, et al. Molecular peritoneal staging for pancreatic ductal adenocarcinoma using mutant KRAS droplet-digital polymerase chain reaction: Results of a prospective clinical trial. J Am Coll Surg. 2021;233(1):73-80.e1. doi:10.1016/j.jamcollsurg.2021.05.009)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

81275-KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene) (eg, carcinoma) gene analysis, variants in codons 12 and 13

81276-KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene) (eg, carcinoma) gene analysis, additional variants