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HelpOrder Code LAB1991 Alpha-Fetoprotein, Amniotic Fluid
Additional Codes
Mayo Test Code: AFPA
Reporting Name
Alpha Fetoprotein, AFUseful For
Screening for open neural tube defects or other fetal abnormalities
Follow-up testing for patients with elevated serum alpha-fetoprotein results or in conjunction with cytogenetic testing
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ACHE_ | Acetylcholinesterase, AF | Yes | No |
Testing Algorithm
If alpha-fetoprotein is positive, then acetylcholinesterase will be performed at an additional charge.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Amniotic FldNecessary Information
The following information is required:
1. Estimated due date by ultrasound
2. Collection date
3. Gestational age must be between 13 and 24 weeks; 16 to 18 weeks preferred.
If not ordering electronically, provide information on Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information (T595) and send with specimen.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Container/Tube: Plain, plastic, screw-top tube
Specimen Volume: 0.75 mL
Collection Instructions: Do not centrifuge.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Amniotic Fld | Refrigerated (preferred) | 7 days | |
| Ambient | 7 days |
Special Instructions
Reference Values
< 2.0 multiples of median (MoM)
Day(s) Performed
Monday through Friday
CPT Code Information
82106
82013 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| AFPA | Alpha Fetoprotein, AF | 58735-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 6739 | Collection Date | 58734-5 |
| DAT15 | EDD by US Scan | 11781-2 |
| 6740 | Last Menstrual Period (LMP) | 8665-2 |
| 24233 | EDD by LMP | 11779-6 |
| 24239 | GA at Collection by Scan | 11888-5 |
| 24240 | GA at Collection by Dates | 11885-1 |
| 24234 | GA Used | 21299-3 |
| 9950 | Alpha Fetoprotein, AF | 1832-5 |
| 24241 | Results | 29595-6 |
| 24235 | Interpretation | 59462-2 |
| 24236 | Additional Comments | 48767-8 |
| 24237 | Follow up | 80615-8 |
| 24238 | General Test Info | 48767-8 |
Method Description
The Access AFP (alpha-fetoprotein) assay is a 2-site immunoenzymatic sandwich assay. A sample is added to a reaction vessel with mouse monoclonal anti-AFP alkaline phosphatase conjugate and paramagnetic particles coated with a second mouse monoclonal anti-AFP antibody. The AFP in the sample binds to the immobilized monoclonal anti-AFP on the solid phase while, at the same time, the monoclonal anti-AFP-alkaline phosphatase conjugate reacts with different antigenic sites on the sample AFP. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. The chemiluminescent substrate Lumi-Phos530 is added to the reaction vessel and light generated by the reaction is measured by a luminometer. The light production is directly proportional to the amount of AFP in the sample. The amount of analyte in the sample is determined by means of a multipoint calibration curve.(Package insert: Access AFP. Beckman Coulter Inc; 12/2021)
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Method Name
AFPA: Immunoenzymatic Assay
ACHE_: Polyacrylamide Electrophoresis
Forms
Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information (T595) is required.