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HelpOrder Code LAB3784 Opiates Confirmation, Random, Urine
Additional Codes
Mayo Code: OPATU
Reporting Name
Opiate Confirmation, UUseful For
Detection and quantification of codeine, hydrocodone, oxycodone, morphine, hydromorphone, oxymorphone, noroxycodone, noroxymorphone, norhydrocodone, dihydrocodeine, and naloxone in urine
Specimen Type
UrineOrdering Guidance
For situations where chain of custody is required, a Chain of Custody Kit (T282) is available. For chain-of-custody testing, order OPATX / Opiates Confirmation, Chain of Custody, Random, Urine.
Additional drug panels and specific requests are available; call 800-533-1710.
Additional Testing Requirements
If urine creatinine is required or adulteration of the sample is suspected, the following test should also be ordered, ADULT / Adulterants Survey, Random, Urine.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Plastic urine container
Submission Container/Tube: Plastic, 5-mL tube
Specimen Volume: 3 mL
Collection Instructions:
1. Collect a random urine specimen.
2. No preservative.
Additional Information:
1. No specimen substitutions.
2. STAT requests are not accepted for this test.
3. Submitting less than 1 mL will compromise our ability to perform all necessary testing.
Specimen Minimum Volume
2.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Urine | Refrigerated (preferred) | 28 days | |
| Frozen | 28 days | ||
| Ambient | 14 days |
Reject Due To
| Gross hemolysis | OK |
| Gross icterus | OK |
Reference Values
Negative
Cutoff concentrations by liquid chromatography tandem mass spectroscopy:
Codeine: 25 ng/mL
Dihydrocodeine: 25 ng/mL
Hydrocodone: 25 ng/mL
Norhydrocodone: 25 ng/mL
Hydromorphone: 25 ng/mL
Oxycodone: 25 ng/mL
Noroxycodone: 25 ng/mL
Oxymorphone: 25 ng/mL
Noroxymorphone: 25 ng/mL
Naloxone: 25 ng/mL
Morphine: 25 ng/mL
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Day(s) Performed
Monday through Friday
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
CPT Code Information
80361
80365
80362
G0480 (if appropriate)
Method Description
Opiates exist in patient urine as both free and either sulfate or glucuronide conjugates. Enzyme hydrolysis is used to liberate the conjugated drug. Specimens are then centrifuged, diluted, and filtered and the analytes are separated by liquid chromatography tandem mass spectroscopy and analyzed by multiple reaction monitoring.(Unpublished Mayo method)
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.