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HelpOrder Code LABBPEH Bordetella pertussis and Bordetella parapertussis, Molecular Detection, PCR, Varies
Additional Codes
Mayo Test Code: BPRPV
EPIC Test Code: LABBPEH
Necessary Information
Specimen source is required.
Specimen Required
The high sensitivity of amplification by polymerase chain reaction (PCR) requires the specimen to be processed in an environment in which contamination of the specimen by Bordetella pertussis or Bordetella parapertussis DNA is unlikely.
Submit only 1 of the following specimens:
Preferred:
Specimen Type: Nasopharyngeal swab
Supplies: Culture Swab - Liquid Stuarts/Single Swab (NP Swab) (T515)
Container/Tube: Rayon swab with an aluminum shaft placed in transport medium such as a green-top nasopharyngeal swab (rayon mini-tip) with Stuart's media (no charcoal), or Stuart's media with charcoal, or Amies media with or without charcoal (Transwab Nasopharyngeal with Charcoal System).
Additional Information:
1. Swab transport containers without charcoal must contain a pledget saturated with either Stuart's or Amies liquid media. Clear semi-solid/solid media is gel and will be rejected.
2. Other swab or media types may be inhibitory to PCR testing and will be rejected.
Acceptable:
Specimen Type: Nasopharyngeal (not throat) aspirate/wash or nasal aspirate/wash
Container/Tube: Sterile container with a screw top cap (no transport media)
Specimen Volume: Entire collection
Useful For
Preferred diagnostic test for the detection of Bordetella pertussis or Bordetella parapertussis
This test is not recommended for screening asymptomatic individuals who may carry B pertussis or parapertussis.
This test is not recommended for follow up of patients previously diagnosed with pertussis (ie, as a test of cure).
Method Name
Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reporting Name
Bordetella, PCR, VariesSpecimen Type
VariesSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Refrigerated (preferred) | 7 days |
| Ambient | 7 days | |
| Frozen | 7 days |
Reject Due To
| Nose, nasal, or throat swab Calcium alginate or cotton-tipped swab Swab sent in gel transport medium, viral/universal transport medium, or Regan Lowe media ESwab Swabs with solid plastic shaft Dry swab |
Reject |
Reference Values
Not applicable
Method Description
The LightCycler instrument platform amplifies and monitors the development of target nucleic acid sequences by fluorescence after each cycle of polymerase chain reaction (PCR). The automated detection of amplified products is based on the fluorescence resonance energy transfer principle. The assay uses the repetitive (50-100 copies) insertion sequence (IS481) found in Bordetella pertussis and the repetitive (35-50 copies) insertion sequence (IS1001) found in Bordetella parapertussis as targets. Detection and differentiation of Bordetella targets is performed through melting curve analysis. The probes were designed to obtain a 10 degree C temperature shift between B pertussis and B parapertussis that is seen in the melting curve analysis. Analysis of the PCR amplification and probe melting curves is accomplished through the use of the LightCycler software.(Sloan LM, Hopkins MK, Mitchell PS, et al. Multiplex LightCycler PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis in nasopharyngeal specimens. J Clin Microbiol. 2002;40[1]:96-100; van der Zee A, Schellekens JF, Mooi FR. Laboratory diagnosis of pertussis. Clin Microbiol Rev. 2015;28[4]:1005-26. doi:10.1128/CMR.00031-15)
Day(s) Performed
Monday through Sunday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87798 x 2
87798 (if appropriate for government payers)
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.