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Order Code LABEHBAP Ehrlichia/Babesia Antibody Panel, Immunofluorescence, Serum

Additional Codes

Mayo Test Code: EHBAP
EPIC Order Code: LABEHBAP


Ordering Guidance


This test may be nonreactive during the acute phase of the infection. For patients presenting with suspected acute infections of Ehrlichia chaffeensis or Anaplasma phagocytophilum, consider EPCRB / Ehrlichia/Anaplasma, Molecular Detection, PCR, Blood.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

As an adjunct in the diagnosis of infection with Anaplasma phagocytophilum, Ehrlichia chaffeensis or Babesia microti

 

Seroepidemiological surveys of the prevalence of the infection in certain populations

Profile Information

Test ID Reporting Name Available Separately Always Performed
ANAP Anaplasma phagocytophilum Ab, IgG,S Yes Yes
EHRC Ehrlichia Chaffeensis (HME) Ab, IgG Yes Yes
BABG Babesia microti IgG Ab, S Yes Yes

Testing Algorithm

For more information see Acute Tick-Borne Disease Testing Algorithm.

Method Name

Immunofluorescence Assay (IFA)

Reporting Name

Ehrlichia/Babesia Ab Panel, S, IFA

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat-inactivated specimen Reject

Reference Values

ANAPLASMA PHAGOCYTOPHILUM

<1:64

Reference values apply to all ages.

 

EHRLICHIA CHAFFEENSIS

<1:64

Reference values apply to all ages.

 

BABESIA MICROTI

<1:64

Reference values apply to all ages.

Method Description

Anaplasma phagocytophilum and Ehrlichia chaffeensis:

The patient's serum is diluted and is placed in microscopic slide wells that have been coated with Anaplasma phagocytophilum or Ehrlichia chaffeensis-infected cells. After incubation, the slides are washed and a fluorescein-isothiocyanate conjugate is added to each well. The slides are then read using a fluorescence microscope and significant fluorescent staining of intracellular organisms constitutes a positive reaction.(Dumler JS, Asanovich KM, Bakken JS, Richter P, Kimsey R, Madigan JE. Serologic cross-reactions among Ehrlichia equi, Ehrlichia phagocytophila, and human granulocytic Ehrlichia. J Clin Microbiol. 1995;33[5]:1098-1103; Pancholi P, Kolbert CP, Mitchell PD, et al. Ixodes dammini as a potential vector of human granulocytic ehrlichiosis. J Infect Dis. 1995;172[4]:1007-1012; Dawson JE, Fishbein DB, Eng TR, Redus MA, Green NR. Diagnosis of human ehrlichiosis with the indirect fluorescent antibody test: kinetics and specificity. J Infect Dis. 1990;162[1]:91-95; package insert: Ehrlichia chaffeensis IFA IgG. DiaSorin; 08/2016)

 

Babesia microti:

The patient's serum is diluted and is placed in microscopic slide wells which have been coated with Babesia microti-infected red blood cells from Syrian hamsters. After incubation, the slides are washed and a fluorescein-isothiocyanate conjugate is added to each well. The slides are then read using a fluorescence microscope and significant fluorescent staining of intraerythrocytic organisms constitutes a positive reaction.(Krause PJ, Telford III SR, Ryan R, et al. Diagnosis of babesiosis: Evaluation of a serologic test for the detection of Babesia microti antibody. J Infect Dis. 1994;169[4]:923-926; package insert: Babesia IFA IgG. DiaSorin Molecular; 08/12/2016)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86666 x 2

86753

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.