Home
HelpOrder Code LABGOR, A2PI Alpha-2 Plasmin Inhibitor, Plasma
Specimen Required
Specimen Type: Platelet-poor plasma
Collection Container/Tube: Light-blue top (3.2% sodium citrate)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.
2. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.
3. Aliquot plasma into a plastic vial leaving 0.25 mL in the bottom of centrifuged vial.
4. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally, -40° C or below.
Additional Information:
1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. Each coagulation assay requested should have its own vial.
Useful For
Diagnosing congenital alpha-2 plasmin inhibitor deficiencies (rare)
Providing a complete assessment of disseminated intravascular coagulation, intravascular coagulation and fibrinolysis, or hyperfibrinolysis (primary fibrinolysis), when measured in conjunction with fibrinogen, fibrin D-dimer, fibrin degradation products, soluble fibrin monomer complex, and plasminogen
Evaluating liver disease
Evaluating the effects of fibrinolytic or antifibrinolytic therapy
Special Instructions
Method Name
Chromogenic
Reporting Name
Alpha-2 Plasmin Inhibitor, PSpecimen Type
Plasma Na CitSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma Na Cit | Frozen | 14 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | Reject |
Reference Values
Adults: 80-140%
Normal, full-term, and premature infants may have mildly decreased levels (≥50%) that reach adult levels within 90 days postnatal.*
*See Pediatric Hemostasis References section in Coagulation Guidelines for Specimen Handling and Processing.
Method Description
This assay is performed using the HemosIL Plasmin Inhibitor Kit on the Instrumentation Laboratory ACL TOP Family. Patient plasma, containing alpha-2 plasmin inhibitor, is mixed with reagent containing excess plasmin. Plasmin activity in the reagent is rapidly inhibited by alpha-2 plasmin inhibitor. Residual plasmin activity is then measured using an amidolytic activity assay, in which residual plasmin lyses a synthetic chromogenic substrate and subsequently releases para-nitroanline (detected at 405 nm) to a level that is inversely proportional to the amount of alpha-2 plasmin inhibitor in the sample.(Teger-Nilsson AC, Friberger P, Gyzander E. Determination of a new rapid plasmin inhibitor in human blood by means of a plasmin specific tripeptide substrate. Scand J Clin Lab Invest. 1977;37(5):403-409; package insert: HemosIL Plasmin Inhibitor. Instrumentation Laboratory; 11/2019)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
85410
Forms
If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.