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HelpOrder Code LABGOR, ALDNA Aldosterone with Sodium, 24 Hour, Urine
Reporting Name
Aldosterone with Sodium, UrineUseful For
Investigating primary aldosteronism (eg, adrenal adenoma/carcinoma and adrenal cortical hyperplasia) and secondary aldosteronism (eg, renovascular disease, salt depletion, potassium loading, cardiac failure with ascites, pregnancy, Bartter syndrome) in conjunction with urine sodium levels
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ALDU | Aldosterone, U | Yes | Yes |
| NAU | Sodium, 24 HR, U | Yes | Yes |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
UrineNecessary Information
24-Hour volume (in milliliters) is required.
Specimen Required
Patient Preparation: Spironolactone (Aldactone) should be discontinued for 4 to 6 weeks before testing. The plasma renin activity cannot be interpreted if the patient is being treated with spironolactone.
Supplies: Sarstedt Aliquot Tube 5mL (T914)
Container/Tube: 2 Plastic, 5-mL tubes
Specimen Volume: 10 mL
Collection Instructions:
1. Add 25 mL of 50% acetic acid as preservative at start of collection. Use 15 mL of 50% acetic acid for children under the age of 5 years. This preservative is intended to achieve a pH of between approximately 2 and 4.
2. Collect urine for a full 24 hours (required) and record the total volume.
3. Place 5 mL of well mixed, 24-hour urine in plastic, 5-mL tube and label as Aldosterone.
4. Place 5 mL of well mixed, 24-hour urine in plastic, 5-mL tube and label as Sodium.
Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens for multiple collections.
Specimen Minimum Volume
2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated (preferred) | 14 days |
| Frozen | 14 days | |
| Ambient | 7 days |
Special Instructions
Reference Values
ALDOSTERONE
0-30 days: 0.7-11.0 mcg/24 h*
1-11 months: 0.7-22.0 mcg/24 h*
≥1 year: 2.0-20.0 mcg/24 h
*Loeuille GA, Racadot A, Vasseur P, Vandewalle B. Blood and urinary aldosterone levels in normal neonates, infants and children. Pediatrie 1981;36(5):335-344
SODIUM
41-227 mmol/24 h
If the 24-hour urinary sodium excretion is greater than 200 mmol, the urinary aldosterone excretion should be less than10 mcg.
Day(s) Performed
Tuesday, Thursday
CPT Code Information
82088-Aldosterone
84300-Sodium
Method Description
Sodium:
The ion selective electron (ISE) module indirectly measures the electromotive force (EMF) difference between an ISE and a reference electrode. The EMF of the ISE is dependent on the ion concentration of the sample. The EMF of the reference electrode is constant. An electronic calculation circuit converts EMF of the sample to the ion concentration of the sample.(Package insert: ISE indirect Na, K, Cl for Gen 2. Roche Diagnostics; V14.0, 02/2018)
Aldosterone:
Samples are spiked with deuterated internal standard and are hydrolyzed overnight with acid. Samples are then neutralized and extracted by solid phase extraction. The extracts are dried, reconstituted, and analyzed by liquid chromatography tandem mass spectrometry.(Taylor RL, Singh RJ. Validation of liquid chromatography-tandem mass spectrometry method for analysis of urinary conjugated metanephrine and normetanephrine for screening of pheochromocytoma. Clin Chem. 2002;48[3]:533-539; Wurth R, Tirosh A, Kamilaris CDC, et al. Volumetric modeling of adrenal gland size in primary bilateral macronodular adrenocortical hyperplasia. J Endocr Soc. 202029;5[1]:bvaa162)
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Method Name
ALDU: Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
NAU: Potentiometric, Indirect Ion-Selective Electrode (ISE)
Forms
If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.