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Order Code LABGOR, CAMPC Campylobacter Culture, Feces

Additional Codes

Ordering Generic Mayo Test, LABGOR and then enter Mayo test code CAMPC


Additional Testing Requirements


If susceptibility testing is needed, also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies. If susceptibility testing is not needed (eg, due to lack of recovery of Campylobacter species from feces), it will not be performed and the ZMMLS order will be canceled at time of report.

 

In some cases, local public health requirements may impact Mayo Clinic Laboratories clients, requiring, for example, submission of isolates to public health laboratories. Clients should familiarize themselves with local requirements and are responsible for submitting isolates to appropriate public health laboratories. Clients can obtain isolates of Campylobacter species recovered from specimens submitted to Mayo Clinic Laboratories by calling 800-533-1710 as soon as possible after reporting (to ensure viability of the bacterium).



Shipping Instructions


Specimen must arrive within 96 hours of collection.



Necessary Information


Specimen source is required.



Specimen Required


Patient Preparation: Patient should not use barium or bismuth for 7 to 10 days before collection of specimen.

Supplies: Culture and Sensitivity Stool Transport Vial (T058)

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: Representative portion of fecal specimen

Collection Instructions:

1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

3. Place vial in a sealed plastic bag.


Useful For

Determining whether Campylobacter species may be the cause of diarrhea

 

Reflexive testing for Campylobacter species from nucleic acid amplification test-positive feces

 

This test is generally not useful for patients hospitalized more than 3 days because the yield from specimens from these patients is very low, as is the likelihood of identifying a pathogen that has not been detected previously.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed at an additional charge.

 

For more information see Laboratory Testing for Infectious Causes of Diarrhea.

Method Name

Conventional Culture

Reporting Name

Campylobacter Culture, F

Specimen Type

Fecal

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Fecal Ambient (preferred) 4 days
  Refrigerated  4 days

Reject Due To

Unpreserved feces
ECOFIX preservative
Formalin or PVA fixative
Specimen in buffered glycerol saline transport media
Reject

Reference Values

No growth of Campylobacter species.

Method Description

The fecal specimen is inoculated onto Campylobacter selective agar and grown in microaerophilic conditions at 42° C. After incubation, suspect Campylobacter colonies are identified using one or a combination of the following techniques: Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, conventional biochemical tests, carbon source utilization, serologic methods, or nucleic acid sequencing of the 16S ribosomal RNA (rRNA) gene. Campylobacter species that may be reported include but are not limited to: Campylobacter jejuni; Campylobacter coli; Campylobacter fetus; Campylobacter helveticus; Campylobacter hyointestinalis; Campylobacter lari; Campylobacter subantarcticus; and Campylobacter upsaliensis.(Pillai DR, Griener T. Culture for Campylobacter and related organisms. In: Leber AL, Church DL, eds. Clinical Microbiology Procedures Handbook. 4th ed. ASM Press; 2016:Section 3.8.2)

Day(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87046-Campylobacter Culture, Stool-with isolation and preliminary examination

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87077-Ident by MALDI-TOF mass spec (if appropriate)