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Order Code LABRPRT1 Rapid Plasma Reagin (RPR) Screen with Reflex to Titer, Serum

Additional Codes

Mayo Test Code: RPRT1

EPIC Test Code: LABRPRT1


Ordering Guidance


This test is for monitoring response to therapy in patients treated for syphilis infection. This test should not be used as a primary diagnostic approach for syphilis. To screen for undiagnosed syphilis infection, order SYPH1 / Syphilis IgG with Reflex, Enzyme Immunoassay, Serum



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Determining the current disease status

 

Monitoring response to therapy for syphilis

 

Aid to diagnose congenital syphilis

 

This test cannot be used for testing spinal fluid specimens.

 

This test is not intended for medical-legal use.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RPRT2 RPR Titer,S No No

Testing Algorithm

If this test is positive, then the rapid plasma reagin titer will be performed at an additional charge.

Method Name

Flocculation/Agglutination

Reporting Name

RPR Screen w/ Reflex to Titer,S

Specimen Type

Serum

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Reference Values

Negative

Reference values apply to all ages.

Method Description

If the rapid plasma reagin (RPR) screen is reactive the RPR titer is performed. The RPR titer test is a macroscopic screening assay done with unheated serum. Reagin reacts with nontreponemal antigen containing colloidal charcoal particles. This reaction results in a visual flocculation of the black particles against the white card background. The test yields a positive or negative result, and all positive samples are titered to determine the highest positive dilution.(Huber TW, Storms S, Young P, et al: Reactivity of microhemagglutination, fluorescent treponemal antibody absorption, Venereal Disease Research Laboratory, and rapid plasma reagin tests in primary syphilis. J Clin Microbiol. 1983 Mar;17[3]:405-409; Kaur G, Kaur P: Syphilis testing in blood donors: an update. Blood Transfus. 2015 Apr;13[2]:197-204)

Day(s) Performed

Monday through Saturday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86592

86593-Rapid Plasma Reagin Titer (if appropriate)

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.