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HelpOrder Code MPHNU Methylphenidate and Metabolite, Random, Urine
Additional Testing Requirements
If urine creatinine is required or adulteration of the sample is suspected, also order ADULT / Adulterants Survey, Random, Urine. For more information, see ADULT / Adulterants Survey, Random, Urine.
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube: Plastic urine container
Submission Container/Tube: Plastic, 5-mL tube
Specimen Volume: 5 mL
Collection Instructions:
1. Collect a random urine specimen.
2. No preservative.
Additional Information:
1. No specimen substitutions.
2. STAT requests are not accepted for this test.
3. Submitting less than 1 mL will compromise the ability to perform all necessary testing.
Useful For
Monitoring urine methylphenidate and ritalinic acid concentrations to assess compliance in patients
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Methylphenidate and Metabolite, USpecimen Type
UrineSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated (preferred) | 10 days |
| Frozen | 28 days |
Reject Due To
| Gross hemolysis | OK |
| Gross icterus | OK |
Reference Values
Negative (Positive results are reported with a quantitative result.)
Cutoff concentrations by liquid chromatography tandem mass spectrometry:
Methylphenidate: 10 ng/mL
Ritalinic Acid: 50 ng/mL
Method Description
The urine sample is centrifuged, diluted with internal standard and clinical laboratory reagent water, and then analyzed by liquid chromatography tandem mass spectrometry.(Unpublished Mayo method)
Day(s) Performed
Monday, Wednesday, Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
G0480
80360 (if appropriate for select payers)
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.