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Order Code MPSER Mucopolysaccharides Quantitative, Serum


Ordering Guidance


This test alone is not diagnostic for a specific mucopolysaccharidosis. Follow-up testing must be performed to confirm a diagnosis.



Necessary Information


1. Patient's age is required.

2. Reason for testing is required.

3. Biochemical Genetics Patient Information (T602) is recommended. This information aids in providing a more thorough interpretation of results. Send information with specimen.



Specimen Required


Patient Preparation: Do not administer low-molecular weight heparin prior to collection.

Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Pediatric: 0.2 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Quantification of dermatan sulfate, heparan sulfate, and keratan sulfate in serum to support the biochemical diagnosis of mucopolysaccharidoses types I, II, III, IV, VI, or VII

Genetics Test Information

This test provides diagnostic testing and monitoring of patients with mucopolysaccharidoses (MPS) types I, II, III, IV, VI, and VII.

 

Accumulation of undegraded glycosaminoglycans (GAG; also known as mucopolysaccharides) leads to progressive cellular dysfunction and results in the typical clinical features seen with this group of disorders.

 

Dermatan sulfate (DS), heparan sulfate (HS), and keratan sulfate (KS) are markers for a subset of MPS.

 

Testing for DS and HS in serum can aid in the diagnosis of MPS types I, II, III, VI, and VII.

 

Testing for KS in serum can aid in the diagnosis of MPS IVA and MPS IVB.

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Mucopolysaccharides Quant, S

Specimen Type

Serum Red

Specimen Minimum Volume

0.2 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 90 days
  Frozen  90 days
  Ambient  14 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

DERMATAN SULFATE

≤300.00 ng/mL

 

HEPARAN SULFATE

≤55.00 ng/mL

 

TOTAL KERATAN SULFATE

≤5 years: ≤1800.00 ng/mL

6-18 years: ≤1500.00 ng/mL

≥19 years: ≤1200.00 ng/mL

Method Description

Serum specimens are diluted and dermatan sulfate (DS), heparan sulfate (HS), and keratan sulfate (KS) are enzymatically digested. The reaction mixture is centrifuged and analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The ratio of the extracted peak area of DS, HS, and KS to internal standard as determined by LC-MS/MS is used to calculate the concentration of DS and HS in the sample.(Unpublished Mayo method)

Day(s) Performed

Twice per month

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83864

Testing Algorithm

For more information see Newborn Screening Follow up for Mucopolysaccharidosis type II