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HelpOrder Code MTBS Microsporidia Stain, Varies
Reporting Name
Microsporidia StainUseful For
Diagnosis of extra-intestinal microsporidiosis involving the lung, skin, and other organs, particularly in immunocompromised hosts
Diagnosis of ocular microsporidiosis
Testing Algorithm
For more information see Parasitic Investigation of Stool Specimens Algorithm
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesOrdering Guidance
This test is intended to be ordered on specimens other than stool and urine.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Duodenal aspirate (small intestinal aspirate, jejunal aspirate, small bowel aspirate)
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Additional Information: ECOFIX and 10% formalin are acceptable preservatives.
Specimen Stability Information: Preserved Ambient (preferred) <10 days/Refrigerated <3 days
Specimen Type: Respiratory secretions (bronchoalveolar lavage [BAL], sputum, bronchial wash, pleural fluid)
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Specimen Stability Information: Refrigerated <3 days (preferred)/Frozen <10 days
Specimen Type: Eye (vitreous fluid, ocular fluid)
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Specimen Stability: Refrigerated <3 days
Specimen Type: Corneal scraping
Container/Tube: Sterile container
Specimen Volume: 0.5mL in sterile saline
Additional Information: Place scrapings in sterile saline for shipping.
Specimen Stability: Refrigerated <3 days
Specimen Type: Fresh tissue (lung, eye, bladder, rectal, intestinal, colon, skin, muscle, kidney)
Container/Tube: Sterile container
Specimen Volume: 3-mm biopsy in 0.1-mL sterile saline
Specimen Stability: Refrigerated <3 days
Specimen Type: Gallbladder aspirate/Bile aspirate
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Specimen Stability: Refrigerated <3 days/Frozen <10 days
Specimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | |
Special Instructions
Reference Values
Negative
If positive, reported as Microsporidia detected
Day(s) Performed
Monday through Friday
CPT Code Information
87015-Concentration
87207-Stain
Method Description
Specimen concentrates are stained by the trichrome-blue method.(DeGirolami PC, Ezratty CR, Desai G, et al. Diagnosis of intestinal microsporidiosis by examination of stool and duodenal aspirate with Weber's modified trichrome and Uvitex 2B stains. J Clin Microbiol. 1995;33:805-810)
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Method Name
Trichrome-Blue Stain (Ryan Modification)
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.