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HelpOrder Code MTBXS Mycobacterium tuberculosis Complex, Molecular Detection and Rifampin Resistance, PCR, Sputum
Additional Testing Requirements
Mycobacterial culture is required for epidemiological strain typing and growth-based phenotypic antimicrobial susceptibility testing, including definitive rifampin results as well as results for other antimicrobials. If your facility is unable to perform a mycobacterial culture, order CTB / Mycobacteria and Nocardia Culture, Varies concurrently with this test.
Shipping Instructions
Specimen must arrive within 7 days of collection; if received greater than 7 days from collection, the specimen will be rejected.
Necessary Information
Specimen source is required.
Specimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Mycobacterium tuberculosis DNA is unlikely.
Specimen Type: Sputum (undigested)
Container/Tube: Sterile container
Specimen Volume: 3 mL
Specimen Stability Information: Refrigerated (preferred) 7 days/Ambient 72 hours
Additional Information:
1. If a single specimen is being shared between mycobacterial culture, acid-fast smear, and/or M tuberculosis PCR, a minimum volume of 3 mL for the respiratory specimen is required. Specimen volumes less than indicated may decrease sensitivity of testing.
2. If insufficient volume is submitted, testing will be canceled.
Specimen Type: N-acetyl-L-cysteine/sodium hydroxide (NALC/NaOH)-digested sputum
Container/Tube: Sterile container
Specimen Volume: 3 mL
Collection Instructions:
1. Submit digested specimen treated with NALC/NaOH.
2. Clearly indicate on container and order form that specimen is a digested specimen.
Specimen Stability Information: Refrigerated 7 days
Additional Information:
1. If a single specimen is being shared between mycobacterial culture, acid-fast smear, and/or M tuberculosis PCR, a minimum volume of 3 mL for the respiratory specimen is required. Specimen volumes less than indicated may decrease sensitivity of testing.
2. If insufficient volume is submitted, testing will be canceled.
Useful For
Rapid detection of Mycobacterium tuberculosis DNA from respiratory specimens for the diagnosis of pulmonary tuberculosis
Presumptive detection of rifampin resistance based on the presence of resistance-associated mutations
Method Name
Real-Time Polymerase Chain Reaction (PCR)
Reporting Name
M tuberculosis/ RIF PCR GeneXpertSpecimen Type
SputumSpecimen Minimum Volume
1.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Sputum | Refrigerated (preferred) | 7 days |
| Ambient | 72 hours |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
Negative
Method Description
The Cepheid Xpert MTB/RIF assay utilizes sputum or decontaminated and pelleted sputum sediment. Specimens are inoculated directly into single disposable test cartridges, which contain both DNA extraction and target amplification material. The assay amplifies a 192 base-pair segment of the rpoB gene for Mycobacterium tuberculosis complex identification as well as rifampin resistance profiling. The Cepheid Xpert MTB/RIF assay is a closed polymerase chain reaction (PCR) system that greatly reduces the potential for false-positive results due to specimen cross-contamination as compared with traditional open-system PCR methods.(Package insert: Xpert MTB/RIF. Cepheid; GXMTB/RIF-US-10, rev D, 03/2016)
Day(s) Performed
Monday through Sunday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
87564
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.