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Order Code NCSRY Nicotine Survey, Serum

Useful For

Monitoring tobacco use in a health fair setting

Profile Information

Test ID Reporting Name Available Separately Always Performed
NICOS Nicotine and Metabolites, S Yes Yes

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Nicotine Survey, S

Specimen Type

Serum Red


Necessary Information


Knowledge of time elapsed between last use and specimen collection is important for interpretation of test results.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.8 mL

Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Refrigerated (preferred) 28 days
  Ambient  28 days
  Frozen  28 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

NICOTINE

<3.0 ng/mL

 

COTININE

<3.0 ng/mL

Method Description

Nicotine and cotinine are extracted from serum. The extract is quantified by high-performance liquid chromatography-tandem mass spectrometry.(Unpublished Mayo method)

Day(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

80323

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.