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HelpOrder Code NFLP Neurofilament Light Chain, Plasma
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: None
Submission Container/Tube: Plastic screw-top vial
Specimen Volume: 0.6 mL
Collection Information: Centrifuge and aliquot plasma into a plastic vial. Do not submit specimen in original tube.
Useful For
Assessing neuronal damage related to various neurodegenerative diseases
Method Name
Chemiluminescent Enzyme Immunoassay
Reporting Name
Neurofilament Light Chain, PSpecimen Type
EDTA PlasmaSpecimen Minimum Volume
0.50 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| EDTA Plasma | Refrigerated (preferred) | 14 days |
| Frozen | 90 days | |
| Ambient | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
Reference Values
<2.5 years: ≤12.8 pg/mL
2.5 to 4 years: ≤11.8 pg/mL
5 to 9 years: ≤10.4 pg/mL
10 to 14 years: ≤8.8 pg/mL
15 to 19 years: ≤9.2 pg/mL
20 to 24 years: ≤10.4 pg/mL
25 to 29 years: ≤11.9 pg/mL
30 to 34 years: ≤13.5 pg/mL
35 to 39 years: ≤15.3 pg/mL
40 to 44 years: ≤17.3 pg/mL
45 to 49 years: ≤19.7 pg/mL
50 to 54 years: ≤22.4 pg/mL
55 to 59 years: ≤25.4 pg/mL
60 to 64 years: ≤28.8 pg/mL
65 to 69 years: ≤32.7 pg/mL
70 to 74 years: ≤37.1 pg/mL
75 to 79 years: ≤42.1 pg/mL
80 to 84 years: ≤ 47.8 pg/mL
≥85 years: ≤54.3 pg/mL
Method Description
Plasma calibrator or specimen are added to particle solution. Neurofilament Light (NfL) in specimens or calibrators specifically binds to anti-NfL monoclonal antibody (mouse) on the particles and antigen-antibody immunocomplexes are formed. The particles are washed and rinsed to remove unbound materials. Alkaline phosphatase (ALP)-labeled anti-NfL monoclonal antibodies (mouse) are added and specifically bind to the prior formed immunocomplexes on the particles, and additional immunocomplexes are formed. The particles are washed and rinsed to remove unbound materials. Substrate solution is added and mixed with the particles and 3-(2'-spiroadamantane)-4-methoxy-4-(3"-phosphoryloxy) phenyl-1,2-dioxetane disodium salt (AMPPD) contained in the substrate solution is dephosphorylated by the catalysis of ALP indirectly conjugated to particles. Luminescence (at a maximum wavelength of 477 nm) is generated by the cleavage reaction of dephosphorylated AMPPD. The luminescent signal reflects the amount of NfL in the sample.(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
83884
Forms
If not ordering electronically, complete, print, and send a Neurology Specialty Testing Client Test Request (T732) with the specimen.