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HelpOrder Code NGPCM MayoComplete Plasma Cell Myeloma, Next-Generation Sequencing, Varies
Shipping Instructions
Bone marrow aspirate samples must arrive within 4 days of collection.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Bone marrow aspirate
Container/Tube: Lavender or pink top (EDTA) or yellow top (ACD)
Specimen Volume: 2 mL
Collection Instructions:
1. Minimum plasma cell percentage is 5%.
2. Invert several times to mix bone marrow.
3. Send bone marrow specimen in original tube. Do not aliquot.
4. Label specimen as bone marrow.
5. Fresh specimen is required for this test, as testing is performed on sorted cells.
Specimen Stability Information: Ambient (preferred) 4 days/Refrigerate
Additional Information: To ensure minimum volume and concentration of DNA are met, the requested volume must be submitted. Testing may be canceled if DNA requirements are inadequate.
Specimen Type: Paraffin-embedded bone marrow clot
Container/Tube: Paraffin block
1. Send 1 hematoxylin and eosin-stained slide in addition to the paraffin block.
2. Minimum plasma cell percentage is 20%.
3. Required amount of tissue area is at least 25 mm(2).
4. Tissue should be fixed in 10% neutral-buffered formalin. Other fixatives are not acceptable.
5. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.
Specimen Stability Information: Ambient
Additional Information: If the quality of the specimen is poor or the plasma cell population is below 20%, testing should not be ordered. Testing may be canceled if DNA requirements are inadequate.
Specimen Type: Tissue slide, bone marrow clot
Slides: 10 unstained slides
Container/Tube: Transport in plastic slide holders.
Collection Instructions:
1. Send 10 unstained, nonbaked slides with 5-micron thick sections of tissue and 1 hematoxylin and eosin-stained slide.
2. Minimum amount of plasma cell percentage is 20%.
4. Required amount of tissue area is at least 25 mm(2).
5. Tissue should be fixed in 10% neutral-buffered formalin. Other fixatives are not acceptable.
6. Decalcified specimens (eg, bone marrow core biopsies) are not acceptable.
Specimen Stability Information: Ambient
Additional Information: Testing may be canceled if resultant extracted DNA does not meet concentration requirements.
Forms
1. Hematopathology Patient Information (T676)
2. If not ordering electronically, complete, print, and send an Hematopathology/Cytogenetics Test Request (T726) with the specimen.
Useful For
Evaluating multiple myeloma at the time of diagnosis and at disease relapse or when changing clinical management to provide prognostic information and determine potential therapeutic implications
Genetics Test Information
This test includes next-generation sequencing to evaluate the following 26 genes and select intronic regions: BIRC3, BRAF, CCND1, CDKN2A, CRBN, CUL4A, CUL4B, CXCR4, DIS3, EGFR, IDH1, IDH2, IKZF1, IKZF3, KRAS, MYC, MYD88, NRAS, NSD2, PIK3CA, PIM1, STAT3, TENT5C, TP53, TRAF3, and XBP1.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CSPMM | NGPCM Pre-Analysis Cell Sorting, BM | No | No |
Testing Algorithm
For a list of the genes and exons targeted by this assay, see Targeted Genes Interrogated by MayoComplete Plasma Cell Myeloma Next-Generation Sequencing.
Special Instructions
Method Name
Next-Generation Sequencing (NGS)
Reporting Name
Plasma Cell Myeloma, NGS, VSpecimen Type
VariesSpecimen Minimum Volume
Bone marrow aspirate: 1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Varies | |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Specimens that have been decalcified (all methods) Bone marrow core biopsies Paraffin shavings Frozen tissue Extracted DNA Moderately to severely clotted bone marrow aspirate |
Reject |
Reference Values
An interpretive report will be provided.
Method Description
This is a laboratory-developed target enriched next generation sequencing (NGS) panel. DNA is extracted from validated specimen sources including bone marrow aspirate and formalin-fixed paraffin embedded tissues (eg, bone marrow clot). Bone marrow aspirate specimens are enriched for plasma cells using a flow cytometric cell sorting selection method prior to DNA extraction. Library preparation for NGS is performed followed by probe hybridization and capture. Sequencing of the final sample library is performed on an NGS instrument. Following bioinformatic processing of the sequencing data, the sequencing results are interpreted to provide a final clinical report. Genomic alterations are called according to human genome reference build GRCh37 (hg19).(Unpublished Mayo method)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
81450