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Order Code SSB SS-B/La Antibodies, IgG, Serum

Additional Codes

Mayo Test ID
SSB

Reporting Name

SS-B/La Ab, IgG, S

Useful For

Evaluating patients with clinical features or at-risk for connective tissue disease, especially Sjögren syndrome.

Specimen Type

Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

0.35 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 21 days
  Frozen  21 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK
Heat-treated Reject

Reference Values

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Day(s) Performed

Monday through Saturday

Method Name

Multiplex Flow Immunoassay

CPT Code Information

86235

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SSB SS-B/La Ab, IgG, S 33613-1

 

Result ID Test Result Name Result LOINC Value
SSB SS-B/La Ab, IgG, S 33613-1

Testing Algorithm

For more information see Connective Tissue Disease Cascade.

Special Instructions

Method Description

Affinity-purified SS-B antigen is coupled covalently to polystyrene microspheres, which are impregnated with fluorescent dyes to create a unique fluorescent signature. SS-B/La antibodies, if present in diluted serum, bind to the SS-B antigen on the microspheres. The microspheres are washed to remove extraneous serum proteins. Phycoerythrin (PE)-conjugated antihuman IgG antibody is then added to detect IgG anti-SS-B/La bound to the microspheres. The microspheres are washed to remove unbound conjugate, and bound conjugate is detected by laser photometry. A primary laser reveals the fluorescent signature of each microsphere to distinguish it from microspheres that are labeled with other antigens, and a secondary laser reveals the level of PE fluorescence associated with each microsphere. Results are calculated by comparing the median fluorescence response for SS-B/La microspheres to a 4-point calibration curve.(Package insert: Bioplex 2200 ANA Screen. Bio-Rad Laboratories; 02/2019)

Forms

If not ordering electronically, complete, print, and send a Kidney Transplant Test Request with the specimen.