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HelpOrder Code TAB Thyroid Autoantibodies Profile, Serum
Reporting Name
Thyroid Autoantibodies Profile, SUseful For
As an adjunct in the diagnosis of autoimmune thyroid diseases: Hashimoto disease, postpartum thyroiditis, neonatal hypothyroidism, and Graves disease
Differentiating thyroid autoimmune disorders from nonautoimmune goiter or hypothyroidism
As a diagnostic tool in deciding whether to treat a patient who has subclinical hypothyroidism
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TPO | Thyroperoxidase Ab, S | Yes | Yes |
| TGAB | Thyroglobulin Antibody, S | Yes | Yes |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Serum RedOrdering Guidance
If thyroglobulin tumor marker testing is desired, order HTG2 / Thyroglobulin, Tumor Marker, Serum.
Specimen Required
Patient Preparation: For 12 hours before specimen collection, patient should not take multivitamins or dietary supplements (eg, hair, skin, and nail supplements) containing biotin (vitamin B7).
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL serum
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
Serum: 0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 7 days |
| Frozen | 30 days | |
| Ambient | 7 days |
Reference Values
THYROGLOBULIN ANTIBODY
<4.0 IU/mL
Reference values apply to all ages.
THYROPEROXIDASE ANTIBODIES
<9.0 IU/mL
Reference values apply to all ages.
Day(s) Performed
Monday through Saturday
CPT Code Information
86376-Thyroperoxidase antibody
86800-Thyroglobulin antibody
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TAB | Thyroid Autoantibodies Profile, S | 87556-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TGAB | Thyroglobulin Antibody, S | 56536-6 |
| TPO | Thyroperoxidase Ab, S | 8099-4 |
Method Description
Thyroglobulin antibody:
The Access Thyroglobulin Antibody II assay (TgAb) is a sequential 2-step immunoenzymatic (sandwich) assay. A sample is added to a reaction vessel with paramagnetic particles coated with the thyroglobulin protein. The serum TgAb binds to the thyroglobulin. After incubation in a reaction vessel, materials bound to the solid phase are held in place by a magnetic field, while unbound materials are washed away. The thyroglobulin-alkaline phosphatase conjugate is added and binds to the TgAb. After the second incubation, materials bound to the solid phase are held in place by a magnetic field, while unbound materials are washed away. Then, the chemiluminescent substrate is added to the reaction vessel and light generated by the reaction is measured with a luminometer.(Package insert: Access Thyroglobulin Antibody II. Beckman Coulter, Inc; 04/2020)
Thyroperoxidase antibody:
The Access TPO (thyroperoxidase) Antibody assay is a sequential 2-step immunoenzymatic (sandwich) assay. A sample is added to a reaction vessel with paramagnetic particles coated with thyroperoxidase protein. The serum TPO antibody binds to the thyroperoxidase. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Protein A-alkaline phosphatase conjugate is added and binds to the TPO antibody. After the second incubation, materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. The chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of TPO antibody in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve. The analyte in the calibrator is traceable to international standard WHO 66/387.(Package insert: Access TPO Antibody. Beckman Coulter, Inc; 06/2020)
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | Reject |
Method Name
Immunoenzymatic Assay