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HelpOrder Code 7AC4 7AC4, Bile Acid Synthesis, Serum
Specimen Required
Patient Preparation:
1. Fasting: 12 hours, required; Fasting morning specimen is preferred.
2. Patient should not be taking bile acid sequestrants for 24 hours prior to collection or statins for 5 days prior to collection.
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge and aliquot 1 mL of serum into a plastic vial.
2. Send specimen frozen.
Useful For
Screening for bile acid malabsorption in patients with irritable bowel syndrome with diarrhea
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
7AC4, Bile Acid Synthesis, SSpecimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 90 days |
| Refrigerated | 72 hours | |
| Ambient | 24 hours |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
≥18 years: 2.5-63.2 ng/mL
Reference values have not been established for patients who are <18 years of age.
Method Description
7 Alpha-hydroxy-cholesten-3-one (7aC4) is extracted from the sample. After addition of a deuterium-labeled 7aC4 internal standard, 7aC4 is measured by liquid chromatography tandem mass spectrometry. (Donato LJ, Lueke A, Kenyon SM, Meeusen JW, Camilleri M: Description of analytical method and clinical utility of measuring serum 7-alpha-hydroxy-4-cholesten-3-one (7aC4) by mass spectrometry. Clin Biochem. 2018;52:106-111)
Day(s) Performed
Monday, Thursday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82542
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| 7AC4 | 7AC4, Bile Acid Synthesis, S | 94866-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 65504 | 7AC4, Bile Acid Synthesis, S | 94866-1 |
Forms
If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Test Request (T728) with the specimen
Testing Algorithm
For more information see Ordering Guide: Bile Acid-Associated Tests.