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HelpOrder Code A124 Alpha-1-Microglobulin, 24 Hour, Urine
Necessary Information
24-Hour volume (in milliliters) is required.
Specimen Required
Container/Tube: Plastic, 5-mL tube
Specimen Volume: 4 mL
Collection Instructions:
1. Collect urine for 24 hours.
2. No preservative.
3. Mix well before taking 4-mL aliquot.
Additional Information: For multiple collections see Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens
Useful For
Assessment of renal tubular injury or dysfunction using 24-hour urine collections
Screening for tubular abnormalities
Detecting chronic asymptomatic renal tubular dysfunction(2)
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| AIM | Alpha-1-Microglobulin, 24 HR, U | No | Yes |
| A1MCR | A1M/Creat Ratio | No | Yes |
| A1MC | Alpha-1-Microglobulin Concentration | No | Yes |
| CRT24 | Creatinine, 24 HR, U | Yes, (Order CTU) | Yes |
Special Instructions
Method Name
AIM, A1MCR: Calculation
A1MC: Immunonephelometry
CRT24: Enzymatic Colorimetric Assay
Reporting Name
Alpha-1-Microglobulin, 24 HR, USpecimen Type
UrineSpecimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Urine | Refrigerated (preferred) | 7 days | |
| Ambient | 7 days | ||
| Frozen | 7 days |
Reject Due To
| Gross hemolysis | Reject |
Reference Values
≥18 years: <23 mg/24 hours
Reference values have not been established for patients who are less than 18 years of age.
Method Description
Alpha-1-Microglobulin:
In an immunochemical reaction, alpha-1-microglobulin present in the urine sample forms immune complexes with anti-alpha-1-microglobulin specific antibodies. These complexes scatter a beam of light passed through the sample. The intensity of the scattered light is proportional to the concentration of alpha-1-microglobulin in the sample. The result is evaluated by comparison with a standard of known concentration.(Package insert: N Alpha-1-Microglobulin. Siemens Healthcare Diagnostics Inc; V5, 05/2018)
Creatinine:
The enzymatic method is based on the determination of sarcosine from creatinine with the aid of creatininase, creatinase, and sarcosine oxidase. The liberated hydrogen peroxide is measured via a modified Trinder reaction using a colorimetric indicator. Optimization of the buffer system and the colorimetric indicator enables the creatinine concentration to be quantified both precisely and specifically.(Package insert: Creatinine plus ver 2. Roche Diagnostics; V 15.0, 03/2019)
Day(s) Performed
Varies
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
83883
Forms
If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.