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Order Code ADMAB ADAMTS 13 Antibody, Plasma


Ordering Guidance


Consider ordering in patients to aid in distinguishing between congenital thrombotic thrombocytopenic purpura (TTP) and acquired autoimmune TTP.



Specimen Required


Patient Preparation:

Fasting: 8 hours, preferred

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Polypropylene plastic vial

Specimen Volume: 1 mL plasma

Collection Instructions:

1. Specimen must be collected prior to replacement therapy.

2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.

3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

4. Aliquot plasma into a separate plastic vial, leaving 0.25 mL in the bottom of centrifuged vial.

5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally, below -40° C.

Specimen Stability Information: Frozen 21 months

Additional Information:

1. Double-centrifuged specimen is critical for accurate results.

2. Each coagulation assay requested should have its own vial.


Forms

If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.

Useful For

Assisting with the diagnosis and monitoring of congenital, immune, or acquired thrombotic thrombocytopenic purpura

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

ADAMTS 13 Antibody, P

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Na Cit Frozen

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

Negative: <12 U/mL

Borderline positive: 12-15 U/mL

Positive: >15 U/mL

Method Description

Testing is performed on the Janus G3 liquid handler and BioTek microplate reader with Gen5 software using the Technoclone Technozym INH kit. The method used is an enzyme-linked immunosorbent assay.

 

A microtiter plate coated with a recombinant form of ADAMTS13 protease (rADAMTS13) in a 96 well polystyrene plate. Diluted patient plasma is incubated in the wells, allowing anti-ADAMTS-13 antibody in the sample to bind to the (rADAMTS13) in the wells. The plate is washed to remove unbound target. Conjugate: POX anti-human ADAMTS13 detection antibody is added to each well and incubated. After incubation, the conjugate is removed by washing. A chromogenic substrate of tetramethylbenzidine (TMB) is incubated and followed by a stopping solution of 2.5% sulfuric acid, to develop a color reaction. The intensity of the color is measured at 450 nm (reference of 620 nm) and is directly proportional to amount of ADAMTS13 antibody in the sample as determined against a reference line using assayed reference plasma.(Package insert: TECHNOZYM ADAMTS 13 INH ELISA, quantitative test for detection of human autoantibodies (IgG) in serum or plasma against ADAMTS13. Technoclone; Rev.023, 09/22/2021; Dekimpe C, Roose E, Kangro K, et al. Determination of anti-ADAMTS-13 autoantibody titers in ELISA: Influence of ADAMTS-13 presentation and autoantibody detection. J Thromb Haemost. 2021;19[9]:2248-2255. doi:10.1111/jth.15297)

Day(s) Performed

Varies

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

83520

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ADMAB ADAMTS 13 Antibody, P 40824-5

 

Result ID Test Result Name Result LOINC Value
623136 ADAMTS 13 Antibody 40824-5
623272 Interpretation 69049-5