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HelpOrder Code AK1 Adenylate Kinase Enzyme Activity, Blood
Specimen Required
Collection Container/Tube:
Preferred: Yellow top (ACD solution B)
Acceptable: Lavender top (EDTA) or yellow top (ACD solution A)
Specimen Volume: 6 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send whole blood specimen in original tube. Do not aliquot.
Useful For
Evaluation of chronic nonspherocytic hemolytic anemia
Method Name
Kinetic Spectrophotometry
Reporting Name
Adenylate Kinase, BSpecimen Type
Whole Blood ACD-BSpecimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood ACD-B | Refrigerated | 20 days |
Reject Due To
| Gross hemolysis | Reject |
| Fully clotted | Reject |
Reference Values
≥12 months: 195-276 U/g Hb
Reference values have not been established for patients younger than 12 months.
Method Description
Adenylate kinase (myokinase) catalyzes the dismutation of adenosine diphosphate (ADP) into adenosine monophosphate and adenosine triphosphate. In this assay, the reverse reaction is measured by following the formation of ADP with pyruvate kinase and lactate dehydrogenase reactions resulting in 1,4-dihydronicotinamide adenine dinucleotide (NADH) being oxidized to nicotinamide adenine dinucleotide (NAD+). The decrease in absorbance that occurs as NADH is oxidized is measured spectrophotometrically at 340 nm by an automated chemistry analyzer.(Beutler E. Red Cell Metabolism. A Manual of Biochemical Methods. 3rd ed. Grune and Stratton; 1984:93-95; van Solinge WW, van Wijk. Enzymes of the red blood cell. In: Rifai N, Horvath AR, Wittwer CT: eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:chap 30)
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82657
Day(s) Performed
Tuesday, Thursday
Forms
If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.