Method Description

The Alpha Defensin Lateral Flow Test Kit is an immunoassay test system comprised of a single use device, a premeasured vial of dilution buffer, a disposable Microsafe tube, and a sample cup.

Each device contains a reagent strip with all the critical components for the assay. Dilution is performed by collecting a sample from an aspirated synovial fluid specimen using the disposable Microsafe tube and adding the sample to the premeasured dilution buffer. Three full, free-falling drops of the diluted sample are then added to the test device to begin the testing process. Cellular material is removed by the first pad. The solution then migrates to the buffering pad and mixes with the gold conjugate that has been labelled with an anti-alpha defensin antibody. The test mixture then migrates across the test line and the control line. A test result line will form if the level of alpha defensin in the sample is greater than the cut-off concentration. A control line will form to confirm that the solution has properly flowed across the device. Results can be read between 10 to 20 minutes.(Package insert: Synovasure Alpha Defensin Lateral Flow Test Kit. Zimmer Biomet; M40004 V 8, 01/2024)