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Order Code AMISO Amylase, Isoenzymes, Serum


Necessary Information


Age and sex of patient are required.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.


Useful For

Ruling out salivary amylase as the cause of elevated serum amylase

Profile Information

Test ID Reporting Name Available Separately Always Performed
AMYSE Amylase, Total, S Yes, (Order AMS) Yes
AMYPA Amylase, Pancreatic, S No Yes
AMYSA Amylase, Salivary, S No Yes

Testing Algorithm

Total and pancreatic amylase are measured in the submitted serum specimen. Salivary amylase is calculated as the difference between the two measured results (salivary amylase = total amylase-pancreatic amylase).

Method Name

AMYSE, AMYPA: Colorimetric Rate Reaction

AMYSA: Calculation

Reporting Name

Amylase, Isoenzymes, S

Specimen Type

Serum

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 30 days
  Frozen  30 days
  Ambient  7 days

Reject Due To

Gross hemolysis Reject

Reference Values

AMYLASE, TOTAL

0-30 days: ≤6 U/L

31-182 days: 1-17 U/L

183-365 days: 6-44 U/L

1-3 years: 8-79 U/L

4-17 years: 21-110 U/L

≥18 years: 28-100 U/L

 

AMYLASE, PANCREATIC

0-<24 months: ≤20 U/L

2-<18 years: 9-35 U/L

≥18 years: 13-53 U/L

 

AMYLASE, SALIVARY

0-<18 years: Not established

≥18 years: ≤86 U/L

Method Description

Total amylase:

The liquid Roche amylase method is an enzymatic colorimetric test using 4,6-ethylidene-(G7) p-nitrophenyl-(G1)-alpha-D-maltoheptaoside (ethylidene-G7PNP), cleaved under the catalytic action of alpha-amylases. The G2PNP, G3PNP, and G4PNP fragments formed are completely hydrolyzed to p-nitrophenol and glucose by alpha-glucosidase. The color intensity of the p-nitrophenol formed is directly proportional to the alpha-amylase activity. It is determined by measuring the increase in absorbance.(Package insert: AMYL2 reagent. Roche Diagnostics; 12/2018)

 

Pancreatic amylase:

After immunoinhibition with antibodies against human salivary alpha-amylase, the amount of pancreatic alpha-amylase in a sample is selectively determined by an enzymatic colorimetric method using the substrate ethylidene-G7PNP.(Package insert: Alpha-Amylase EPS Pancreatic. Roche Diagnostics; 05/2019)

Day(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

82150 x 2

Forms

If not ordering electronically, complete, print, and send Gastroenterology and Hepatology Test Request (T728) with the specimen.