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Order Code B2MU Beta-2 Microglobulin, Random, Urine


Specimen Required


Patient Preparation: For 12 hours before specimen collection, do not take multivitamins or dietary supplements containing biotin (vitamin B7) which is commonly found in hair, skin, and nail supplements and multivitamins.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Container/Tube: Plastic, urine tube

Specimen Volume: 1.0 mL

Collection Instructions:

1. Patient should empty bladder.

2. Have patient drink at least 0.5 liters of water.

3. Within 1 hour, collect a random urine specimen.

4. Add 1 M sodium hydroxide (NaOH) as preservative to the collection. This preservative is intended to achieve an approximate pH of between 6 and 8.


Useful For

Evaluation of renal tubular damage

 

Monitoring exposure to cadmium and mercury

Method Name

Automated Chemiluminescent Immunometric Assay

Reporting Name

Beta-2 Microglobulin, U

Specimen Type

Urine

Specimen Minimum Volume

0.5mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Frozen (preferred) 14 days
  Refrigerated  48 hours

Reject Due To

Specimen with pH <6 Reject

Reference Values

≤300 mcg/L

Method Description

Testing is performed on the Immulite 2000. The Immulite 2000 Beta-2 Microglobulin assay is a solid phase, 2-site chemiluminescent enzyme-labeled immunometric assay. The solid-phase bead is coated with an affinity-purified murine monoclonal anti-beta-2 antibody. The serum sample and alkaline phosphatase conjugated affinity-purified goat polyclonal anti-beta-2 antibody are incubated to bind beta-2 microglobulin into an antibody sandwich complex.

 

The chemiluminescent substrate, a phosphate ester of adamantyl dioxetane, in the presence of alkaline phosphatase produces light proportional to the concentration of the beta-2 microglobulin in the sample.(Package insert: IMMULITE 2000 Beta-2 Microglobulin. Siemens Healthcare Diagnostics; 03/15/2018)

Day(s) Performed

Monday, Wednesday, Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

82232

Forms

If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.