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HelpOrder Code BAPS Bile Acid Profile, Serum
Ordering Guidance
This test is intended for use by research scientists. Approval must be obtained before ordering.
Specimen Required
Patient Preparation: Patient must be fasting for 12 to 14 hours.
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Evaluating the enterohepatic cycle consisting of the biliary system, intestine, portal circulation, and hepatocytes
Supporting researchers in need of free and conjugated values of all 20 bile acid species as well as total bile acid
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Bile Acid Profile, SSpecimen Type
SerumSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 90 days | |
| Ambient | 90 days | ||
| Frozen | 90 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
Reference Values
Chenodeoxycholic acid: ≤2.26 nmol/mL
Cholic acid: ≤2.74 nmol/mL
Deoxycholic acid: ≤2.84 nmol/mL
Dihydroxycholestanoic acid: ≤0.07 nmol/mL
Glycochenodeoxycholic acid: ≤5.14 nmol/mL
Glycocholic acid: ≤2.17 nmol/mL
Glycodeoxycholic acid: ≤3.88 nmol/mL
Glycohyodeoxycholic acid: ≤0.01 nmol/mL
Glycolithocholic acid: ≤0.11 nmol/mL
Glycoursodeoxycholic acid: ≤1.00 nmol/mL
Hyodeoxycholic acid: ≤0.12 nmol/mL
Lithocholic acid: ≤0.09 nmol/mL
Taurochenodeoxycholic acid: ≤0.80 nmol/mL
Taurocholic acid: ≤0.31 nmol/mL
Taurodeoxycholic acid: ≤0.78 nmol/mL
Taurohyodeoxycholic acid: ≤0.02 nmol/mL
Taurolithocholic acid: ≤0.04 nmol/mL
Tauroursodeoxycholic acid: ≤0.05 nmol/mL
Trihydroxycholestanoic acid: ≤1.73 nmol/mL
Ursodeoxycholic acid: ≤0.64 nmol/mL
Total bile acids: ≤19.00 nmol/mL
Method Description
Bile acid concentrations in serum are measured by liquid chromatography tandem mass spectrometry stable isotope dilution analysis. Serum is mixed with isotopically labeled internal standards of selected bile acids and then subjected to protein precipitation. Sample preparation is semiautomated using a liquid handler. Reverse-phase liquid chromatography is performed to separate free bile acids, their respective tauro- and glyco-conjugates, and 2 bile acid precursors.(Unpublished Mayo method)
Day(s) Performed
Monday through FridayPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
82542