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HelpOrder Code BILHA Schistosoma species Antibody, IgG, Serum
Useful For
Detection of antibodies to Schistosoma species
Method Name
Enzyme Immunoassay (EIA)
Reporting Name
Schistosoma Ab, IgG, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 30 days | |
| Frozen | 30 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Heat inactivated | Reject |
Reference Values
Negative
Method Description
The qualitative immunoenzymatic determination of IgG-class antibodies against Schistosoma mansoni is based on the enzyme-linked immunosorbent assay (ELISA) technique.
Microtiter strip wells are precoated with Schistosoma mansoni antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material, horseradish peroxidase-labelled protein A conjugate is added. This conjugate binds to antigen-antibody complexes. The immune complex formed by the bound conjugate is visualized by adding tetramethylbenzidine substrate, which gives a blue reaction product.
The intensity of this product is proportional to the amount of Schistosoma-specific IgG antibodies in the specimen. Sulfuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450 nm is read using an ELISA microwell plate reader.(Package insert: NovaLisa Schistosoma mansoni, Gold Standard Diagnostics; V SCHG0410engl,dt,fr,it,es18082009-JH Rev 01)
Day(s) Performed
Tuesday, Thursday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86682
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.