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HelpOrder Code BRAZX Brazil Nut Component, IgE, Serum
Specimen Required
Only orderable as a reflex. For more information see BRAZR / Brazil Nut, IgE, with Reflex to Brazil Nut Component, IgE, Serum
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.6 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Evaluation of patients with suspected Brazil nut allergy to component Ber e 1
Testing Algorithm
If the Brazil nut specific total IgE result is 0.10 kU/L or more, then the Brazil nut component (Ber e 1) test is performed at an additional charge.
Method Name
Only orderable as a reflex. For more information see BRAZR / Brazil Nut, IgE, with Reflex to Brazil Nut Component, IgE, Serum.
Fluorescent Enzyme Immunoassay (FEIA)
Reporting Name
Brazil Nut Component, IgE, SSpecimen Type
SerumSpecimen Minimum Volume
0.4 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 90 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
Only orderable as a reflex. For more information see BRAZR / Brazil Nut, IgE, with Reflex to Brazil Nut Component, IgE, Serum
|
Class |
IgE kU/L |
Interpretation |
|
0 |
<0.10 |
Negative |
|
0/1 |
0.10-0.34 |
Borderline/Equivocal |
|
1 |
0.35-0.69 |
Equivocal |
|
2 |
0.70-3.49 |
Positive |
|
3 |
3.50-17.4 |
Positive |
|
4 |
17.5-49.9 |
Strongly positive |
|
5 |
50.0-99.9 |
Strongly positive |
|
6 |
≥100 |
Strongly positive |
Concentrations of 0.70 kU/L or more (class 2 and above) will flag as abnormally high.
Reference values apply to all ages.
Method Description
Specific IgE from the patient's serum reacts with the allergen of interest, which is covalently coupled to an ImmunoCAP. After washing away nonspecific IgE, enzyme-labeled anti-IgE antibody is added to form a complex. After incubation, unbound anti-IgE is washed away, and the bound complex is then incubated with a developing agent. After stopping the reaction, the fluorescence of the eluate is measured. Fluorescence is proportional to the amount of specific IgE present in the patient's sample (ie, the higher the fluorescence value, the more IgE antibody is present).(Package insert: ImmunoCAP System Specific IgE FEIA. Rev 06/2020)
Day(s) Performed
Monday through Friday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
86008